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-80℃
Alpha Synuclein Oligomer
大量
StressMarq
100 µg
产品名称 | Alpha Synuclein 蛋白 |
产品描述 | 重组人 Alpha Synuclein 寡聚体 (动态学稳定的) |
应用范围 | WB, Native PAGE, In vivo assay, In vitro assay |
浓度 | 各批次不同,请详见说明书 |
标记物 | 无标签 |
性质 | 重组 |
来源物种 | 人 |
表达系统 | 大肠杆菌 (E. coli) |
氨基酸序列 | MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA |
纯度 | >95% |
蛋白长度 | 全长蛋白 |
储存缓冲液 | PB pH 7.4 (10 mM KH2PO4, 7.5 mM NaOH, pH 7.4) |
储存温度 | -80ºC |
运输温度 | 干冰. 运输: 该产品会与其他同订单产品分开发货. |
纯化方式 | 离子交换层析纯化 |
Protein Size | 650-1200 kDa |
引用该产品 | Human Recombinant Alpha Synuclein Oligomers (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-484) |
分析证书 | 该蛋白已经通过native-PAGE检测证明纯度大于95%. |
其他相关信息 | 动态学稳定的α突触核蛋白寡聚体由重组单体直接生成,没有添加任何诱导剂/抑制剂,在37℃下可以保持可溶性寡聚体状态至少两周, 并且对多巴胺神经 |
别名 | Alpha synuclein protein, Alpha-synuclein oligomer, Alpha synuclein protein oligomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein oligomers, Alpha Synuclein Protein Oligomers, SYN protein, Parkinson's disease familial 1 Protein |
研究领域 | 神经纤维缠结&Tau, 突触核蛋白, 神经生物学, 神经衰退疾病, 帕金森氏病, 阿尔茨海默病 |
细胞定位 | 细胞核, 细胞质, 膜 |
Accession Number | NP_000336.1 |
GeneID | 6622 |
Swiss Prot | P37840 |
科研背景 | Our kinetically stable oligomers of alpha-synuclein are generated without an inducer or inhibitor and remain stable for at least 2 weeks at 37oC. They present as globular structures under TEM, demonstrate toxicity in rat primary dopaminergic neurons and induce Parkinson’s-associated alpha synuclein phosphoserine 129 pathology. These oligomers have been previously characterized as globular, cylindrical structures with a beta-sheet structure intermediate between monomers and fibrils, and were demonstrated to have a higher toxicity to neurons than alpha-synuclein fibrils (1,2). Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (3). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (4). Functionally, it has been shown to significantly interact with tubulin (5), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (6). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer’s disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (7, 8). |
参考资料 | 1. Chen, S.W., et. al. (2015). PNAS. E1994-E2003. 2. Lorenzen, N., et. al. (2014). JACS. 136: 3859-3868. 3. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 4. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 5. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 6. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 7. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 8. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. |
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