- ￥5002
- Agrisera
- 瑞典
- AS01 013
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
AMBP | Alpha-1-microglobulin (clone BN11.10)
- 规格：
50ug
| Immunogen |
| human and rat alpha-1-microglobulin, native, full length, purified from urine, UniProt: P02760 cleavege product no. PRO_0000017886 |
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Host Mouse
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Clonality Monoclonal
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Subclass/isotype
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| IgG2a; |
|
Format Lyophilized
|
|
Quantity
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| 50 µg |
|
Reconstitution For reconstitution add 50 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
|
|
Tested applications ImmunoAffinity chromatography (IAC), ELISA (ELISA), Immunohistochemistry (IHC), immunoflourescence (IF), Immunoprecipitation (IP),, Radioimmunoassay (RIA), Western blot (WB)
|
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Recommended dilution 0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF), 1 : 2000 (WB)
|
|
Expected | apparent MW
|
| 27 kDa |
名称：AMBP | Alpha-1-microglobulin (clone BN11.10)
货号：AS01 013
品牌：Agrisera
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文献和实验Construction of cDNA Libraries in gt10 or gt11
that it is possible to divide the mRNA population into three classes with most of the mRNAs (11,300) being in the lowest abundance class and making up 39% of the polysomal mRNA. To obtain a cDNA library that contains at least one clone for each mRNA of this class
该词来源于希腊文的 Kλωγ，原义为小枝之集聚，但后来则指由无性繁殖所产生的同基因型的生物群，这是于 1903年由 H． J． Webber最早应用于生物学上的。过去曾译作具有繁殖意思的营养系或含有原语源意思的分支系（群），而现在则多按其原音直接称为克隆。现在该词在个体、细胞和基因等三方面都使用，而且任何一方面似乎都意味着来自同源的犹如复制一样的同一生物群体。（ 1）关于个体：由无性繁殖所增殖的种群均是克隆，这在植物方面，通过体细胞培养，在试管内繁殖、分化可以重新生出完整
Single Clone Excision Protocol
tubes.Add 10 ml of phage supernatant to one,and 1 ml to the other (up to 100ml may be used if the excision hasn't gone well,but usually 1 ml is enough.) 10.Incubate tubes at 37℃ for 15 minutes. 11.Normally,the titer of transfected cells is so high
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