- ￥3805
- Agrisera
- 瑞典
- AS04 042S
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
PsaC | positive control/quantitation standard
- 规格：
100ul
| Format Lyophilized in glycerol |
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Quantity 100 µl
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Reconstitution For reconstitution add 95 µl of sterile water. Note that due to glycerol in buffer, the lyophilized product appears as a dense liquid rather than a powder. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently. Avoid vigorous vortexing, as buffer contains detergent. Upon reconstitution, this standard is ready-to-load and does not require any additions or heating. See additional Handling Instructionsbelow.
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| PsaC standard protein concentration: 0.10 pmol/µl. |
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution Positive control: a 2 μL load per well is optimal for most chemiluminescent detection systems.
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| Standard curve: 3 loads are recommended (eg. 0.5, 2 and 4μL). For most applications a sample load of 0.2 μg of chlorophyll will give a PsaC signal in this range. Exact loads can vary with the sensitivity of your system and the abundance of the target protein in your samples. |
| Note: Optimal quantitation is achieved using moderate sample loads/well, generally 1 to 5 ug total protein. |
| A trial experiment may be required |
| i) to bring your sample load within the standard curve range and |
| ii) to obtain a signal that is strong enough to reliably quantify but not so strong as to consume ECL reagents too quickly or saturate your detection system. These goals may achieved by adjusting both sample and standard loads. |
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Expected | apparent MW
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| 11.5 kDa (larger than native protein due to the addition of His-tag). In most gels PsaC migrates between 9 and 14 kDa |
名称：PsaC | positive control/quantitation standard
货号：AS04 042S
品牌：Agrisera
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
if aliquoted. Do not mix before use. Positive control uses 10ng supercoiled plasmid END LABELLED PROBE PREPARATION 1) Digest plasmid w/ 5''overhang enzyme 2) P/CHCl3 ext 3) EtOH ppt 4) Redissolve to 0.5mg/ml in H2O 5) Start
START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
Stewart Laboratory Standard Operating Procedure
x g, 1700 rpm, 5 min in 15 ml tubes - no faster!), and resuspend in 10 mL 70% cold EtOH. Store at -20°C overnight or for several days Day 2 Pellet cells and wash once with wash buffer (PBS + 0.5% IFS). Pellet and resuspend in 0.5 mL 2M HCl
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