- ￥5250
- Agrisera
- AS06 150
- 美国
- 2025年07月16日
- western blot (WB)
- 兔
- 植物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
兔抗叶绿体外膜通道蛋白 ToC多克隆抗体
- 抗体英文名：
Toc75|chloroplast outer envelope mebrane translocon complex OEP75 protein
- 浓度：
见说明书
- 应用范围：
western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
200 µl
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Toc75|chloroplast outer envelope mebrane translocon complex OEP75 protein介绍：
货 号：AS06 150
中文名称：兔抗叶绿体外膜通道蛋白 ToC多克隆抗体
英文名称：Toc75|chloroplast outer envelope mebrane translocon complex OEP75 protein
应用：western blot (WB)
规格：200 µl
价格：5250元
Toc75|chloroplast outer envelope mebrane translocon complex OEP75 protein简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1 : 1000 - 1: 5000 with standard ECL (WB) |
|
| expected | apparent MW | 89 kDa (with transit peptide) | 75 kDa (for Arabidopsis thaliana) |
|
| confirmed reactivity | Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum |
|
| predicted reactivity | Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Citrus clementina, Citrus sinensis, Coffea canephora, Eucalyptus grandis, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Ricinus communis, Solanum lycopersicum, Solanum tuberosum,Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Populus sp., Prunus persica, Zea mays, |
|
| not reactive in | Chlamydomonas reinhardtii |
|
| selected references | Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Wang et al. (2014). Maintenance of chloroplast structure and function by overexpression of the OsMGD gene leads to enhanced salt tolerance in tobacco. Plant Physiol. 2014 May 19. pii: pp.114.238899. Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence) |
|
| application example 10 µg protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloplast envelope membranes, and (3) thylakoids were separated on 4-12%NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 (AS06 150, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (Invitrogen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). |
|
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文献和实验The Chloroplast Protein Import Machinery: A Review
and inner envelope. This process is mediated by complex molecular machines in the outer (Toc complex) and inner (Tic complex) envelope of chloroplasts, respectively. Most proteins destined for the compartments inside the chloroplast contain a cleavable N
Reconstitution of the SecY Translocon in Nanodiscs
Secretory proteins are transported across the bacterial envelope using a membrane protein complex called the SecY channel or translocon. Major advances in understanding this transporter have been accomplished with methods including
Most chloroplast proteins are synthesized in the cytosol as preproteins with N-terminal cleavable transit peptides and are imported into the organelle through the TOC–TIC translocon system. Import involves a complex set of recognition
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