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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
SAL1 | Sal1 phosphatase
- 规格:
100ul
| Immunogen |
| Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (At5g63980, protein Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005 |
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Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 100 µl
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Reconstitution For reconstitution add 100 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 1000 (WB)
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Expected | apparent MW
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| 37.5 | 41 kDa (Arabidopsis thaliana) |
名称:SAL1 | Sal1 phosphatase
货号:AS07 256
品牌:Agrisera
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文献和实验Purification of acidic phosphatase from mustard seedlings
phosphatases are widely distributed in many organisms, including plants. They work optimally at ~ pH 5 without additional cofactors. The enzymes are classified as E.C. 3.1.3.2. In this experiment, we will extract an acidic phosphatase from seedlings
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
sections in DPX Results Acid phosphatase activity - Red
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