- ￥1440
- Agrisera
- AS10 307S
- 美国
- 2025年07月12日
- Western blot (WB)
- 兔
- 植物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
泛素标准品
- 抗体英文名：
UBQ11|Ubiquitin protein|positive control
- 浓度：
见说明书
- 应用范围：
Western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
50 µl
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UBQ11|Ubiquitin protein|positive control介绍：
货 号：AS10 307S
中文名称：泛素标准品
英文名称：UBQ11|Ubiquitin protein|positive control
应用：Western blot (WB)
规格：50 µl
价格：1440元
UBQ11|Ubiquitin protein|positive control简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | positive control: a 2.5-5 μl load per well is optimal for most chemiluminescent detection systems. |
|
| expected | apparent MW | 10-12 kDa |
|
| not reactive in | no confirmed exceptions from predicted reactivity currently known |
|
| additional information | Concentration: 0.8 µg/µl. Recommended load per gel to allow Coomasie staining and western blot detection is 2.5-5 µl. 1x SDS Loading Buffer contains:
|
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| selected references | to be added when available |
|
application example
|
5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL. |
|
| Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
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文献和实验【资源】Protein Degradation: Ubiquitin and the Chemistry of Life
---------------------------------------------------------------------------- Book Description: The first volume in a new series dedicated to protein degradation, this book lays the foundations of targeted protein breakdown via the ubiquitin pathway. The outstanding importance of the ubiquitin
The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS)
signal response pathways, intracellular sorting and movement of proteins and membrane vesicles, cell-to-cell protein transport, and many others. Here we provide a detailed protocol for the mating-based split-ubiquitin system (mbSUS), which is a sensitive
Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts
employed in these extracts to test the putative ubiquitination and degradation of a protein. Moreover, we describe a detailed practical procedure to test the role of different regulators in the ubiquitin-dependent degradation pathway of a specific protein
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