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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated
- 规格:
0.5mg
| Immunogen |
| Purified human IgG |
|
Host Goat
|
|
Clonality Polyclonal
|
|
Purity Affinity purified goat IgG
|
|
Format Lyophilized
|
|
Quantity 0.5 mg
|
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Reconstitution For reconstitution add 0.55 ml of sterile water
|
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Storage Store lyophilized material at 2-8°C.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
|
|
Recommended dilution The optimal working dilution should be determined by the investigator.
|
名称:Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated
货号:AS10 810
品牌:Agrisera
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
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