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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Penicillin V, Serum (0.1 ml)
- 规格:
0.1ml
| Immunogen penicillin V |
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Host Rabbit
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Clonality Polyclonal
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Purity serum
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Format Liquid
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Quantity 0.1 ml
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Storage 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.
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Tested applications ELISA (ELISA)
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Recommended dilution To be determined by end user.
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Expected | apparent MW n.a.
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名称:Penicillin V, Serum (0.1 ml)
货号:AS11 1661
品牌:Agrisera
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文献和实验Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
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