Gaeumannomyces graminis (total

IgG

佚名     　　IgG主要由脾、淋巴结中的浆细胞合成和分泌，以单体形式存在。在个体发育过程中机体合成IgG的年龄要晚于 IgM，在出生后第3个月开始合成，3～5岁接近成年人水平。IgG是血清中主要的抗体成分，约占血清总Ig的75%。根据IgG分子中γ链抗原性差异，人IgG有4个亚类：IgG1、IgG2、IgG3和IgG4（小鼠4个亚类是IgG1、IgG

RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue

. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 0.1-2.5 mg frozen ground tissue or fresh tissue to a 0.5 mL microtube containing 100ul RCL Buffer. Homogenize throughly using a microfuge tube pestle

Purification of mAb (IgG)

(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10