Goat anti-Human kappa light ch
ain, DyLight® 650 conjugated, min. cross-reactivity to Mouse serum- ￥4168
- Agrisera
- 瑞典
- AS12 2295
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Goat anti-Human kappa light chain, DyLight® 650 conjugated, min. cross-reactivity to Mouse serum
- 规格：
0.5mg
| Immunogen |
| Purified Human Kappa (к) Chain |
|
Host Goat
|
|
Clonality Polyclonal
|
|
Purity Affinity purified goat IgG
|
|
Format Lyophilized
|
|
Quantity 0.5 mg
|
|
Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
|
|
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Recommended dilution 1 : 20-1 : 2000 for most applications
|
名称：Goat anti-Human kappa light chain, DyLight® 650 conjugated, min. cross-reactivity to Mouse serum
货号：AS12 2295
品牌：Agrisera
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
Technovit® 9100 Methyl Methacrylate
with buffer (see above) DAKO EnVision polyvalent antibody (goat-anti-mouse/goat-anti-rabbit) coupled to alkaline phosphatase 30 min at room temperature Visualization Step
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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