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- 供应商:
无锡云萃生物
- CAS号:
1270921-80-6
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5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验5030) CO2 cell culture incubator 50 ml Nunc/Falcon tubes 15 ml Nunc/Falcon tubes KCl (0.075 M, 0.055% ?) Fixative (methanol/acetic acid 3 : 1) glass microscopy slides Amounts per 5 ml blood: 40 ml RPMI 1640 Medium 10 ml FCS (20%)
In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
Ethanol precipitation of nucleic acids
minutes also works.--Heather In general, the time you need to incubate in the freezer depends on how much nucleic acid you have, how big it is and the volume it is in. My general protocol is to freeze for 20 min to 1 hr at -80 ˚C
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