[名称首字母] P
[载体宿主] 哺乳细胞
[原核抗性] Amp
[克隆菌株] DH5α 等
[高拷贝/低拷贝] 高拷贝
[启动子] CMV
载体基本信息
出品公司:Invitrogen
载体名称:pHOOK1
质粒类型:哺乳动物表达载体;Capture-Tec系统;免疫磁珠
高拷贝/低拷贝:高拷贝
克隆方法:限制性内切酶,多克隆位点
启动子:CMV
载体大小:6061 bp
5' 测序引物及序列:CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’
3' 测序引物及序列:SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签:IgK、HA tag (N-term);2XMyc tag (N-term)
载体抗性:氨苄青霉素
筛选标记:博来霉素
克隆菌株:DH5α 等
宿主细胞(系):哺乳动物细胞
备注:
pHOOK1载体可以用于:
1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products
2. The acute effects of constitutive mutations
3. The effect of anti-sense RNA or ribozymes
4. Temporal events along a developmental or signaling pathway
5. Activation or inactivation of downstream genes using differential display
6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for:
a) Immunoblot analysis of proteins
b) Isolation of DNA or RNA
c) Flow Cytometry
See pHOOK-3
产品目录号:V690-20
稳定性:瞬表达
组成型/诱导型:组成型
病毒/非病毒:非病毒