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| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
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文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
. Prepare cell suspensions also in distilled water or 0.05 M KPi with no hexane as controls. Measure the enzyme activity utilizing the Nesslerization reaction. This method utilizes the determination of ammonia liberated from L-asparagine
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
Prepare just before use. Appendix 2 (A2): Reagents Gentamicin sulfate, solution G-1522 Sigma L-glutamin 20 mM , 200 ml 25030-024 Gibco BRL FCS A-1111-L Hyclone EDTA disodium salt
) for 45 minutes at RT (or other conditions, see note 2) in dark, humid chamber; Wash at least 3 times in 1X PBS for 5 minutes each; Incubate with secondary antibody (diluted in same solution as aboved) for 40 minutes at RT in dark and humid
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