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- 供应商:
无锡云萃生物
- CAS号:
13878-92-7
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5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule
Polysialic acid capsules are the major virulence factors in Escherichia coli K1, K92, and groups B and C meningococci. The sialic acid monomers (2-keto-3-deoxy-5-acetamido-7,8,9-d -glycero -d -galacto -nonulosonic acids) comprising
This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.1. Solutions(1) Affigel Blue Prewash0.1 M acetic acid 5.7 ml glacial acetic acid1.4 M NaCl 81 g NaCl40% isopropanol 400 ml isopropanolup
Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
mixture was denatured for 7 min at 95°C and subjected to 28 cycles each with a profile of 60 s at 92°C, 80 s at 52°C and 105 s at 72°C in a DNA Thermal Cycler (Perkin-Elmer, Norwalk, CT, USA). The electrophoresis of the PCR product was performed on a 1
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