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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1241725-60-9
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
buffer; 1ml of ChIP high salt buffer; 1ml of ChIP wash buffer; (note 4)Pulldown (2), Flag-tag specific After the last wash elute the complex by adding 100µl of ChIP elution buffer. Incubate the samples at 65°C for 10 minutes
as given below: Low salt wash buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH8.0, 150 mM NaCl); High salt wash buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH8.0, 500 mM NaCl); LiCl buffer (0.25 M LiCl, 1% NP-40, 1% SDC
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