- ￥100
- ycextract
- 国产/进口
- YD50260
- 2025年07月15日
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- 文献和实验
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- 保存条件：
咨询
- 保质期：
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- 英文名：
N
- 库存：
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- 供应商：
无锡云萃生物
- CAS号：
30①8-12-0
- 规格：
5mg/50mg/100mg/500mg
| 规格： | 5mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥100.0 |
| 规格： | 100mg | 产品价格： | ￥100.0 |
| 规格： | 500mg | 产品价格： | ￥100.0 |
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文献和实验In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
with the peptides) and other (buffer) contaminants (which may give a higher background during MS measurement) Wash the sample 5 min in 80 µl 0.1M NH4HCO3 Add an equal volume of 100% Acetonitrile and vortex for 5 min Spin down the gel
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
4. In-Gel digestion with Trypsin Precool tubes with the dried gel particles and the Trypsin solution on ice Rehydrate the gel particles in the trypsin solution (12.5 ng/µl Trypsin [Promega #V5111] in 50 mM NH4HCO3) for 30-45 minutes at 4°C. Add
sufficient times with 50 µL of a 50%/50% solution of acetonitrile/50mM ammonium bicarbonate to remove all stain from gel plug. Each wash should be approximately 30 minutes in length. Two washes should be sufficient for moderately intensity bands. Wash
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