1,2-Bis(phenylsulfinyl)ethanep

碱裂法提取BAC

of Solution I (10 mM EDTA, pH 8.0; R/T) to every sample and pipette until the pellet is re-suspended.Let them sit for 5 minutes. Solution II: 0.2 M NaOH, 1% SDS (250 ml-> 5 ml 10 M NaOH, 25 ml 10% SDS, and 220 ml water) 4. Add 40 ml of Solution II (prepared

Preparation of BAC DNA with by Alkaline Prep Method

until the pellet is re-suspended. 4. After all the pellets have been re-suspended (go back to 1st bottle, re-pipette 1-2x to look for clumps), move the bottles from the ice to the bench top. Let them sit for 5 minutes. Solution II: 0.2 M NaOH, 1% SDS (250 ml

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially