The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:100
IP
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded human gastric cancer using HDAC2 antibody.
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody.
Immunofluorescence analysis of A549 cells using HDAC2 antibody.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody.
Background:
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.