The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
1:50 - 1:200
IHC
1:50 - 1:500
Immunofluorescence analysis of HeLa cells using GJA5 antibody.
Background:
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.