The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:100
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ACADS antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using ACADS antibody.
Immunohistochemistry of paraffin-embedded human liver injury using ACADS antibody.
Immunofluorescence analysis of U2OS cells using ACADS antibody.
Background:
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.