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- 文献和实验
- 技术资料
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- 英文名:
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- 供应商:
无锡云萃生物
- CAS号:
105694-44-8
- 规格:
100g/500g/1kg/5kg
| 规格: | 100g | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 500g | 产品价格: | ¥100.0 |
| 规格: | 1kg | 产品价格: | ¥100.0 |
| 规格: | 5kg | 产品价格: | ¥100.0 |
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文献和实验Any chromatographic procedure for the separation of phosphoinositols will require a supply of standards, both for validation of the technique and identification of the products. Tritium-radiolabeled standards are commercially available in 1–
Proliferation Assay: [3H] Thymidine incorporation
cells for 2-4 hours then treat. 5) Six-twenty four hours before cells are to be harvested, add 1 µCi/ml [3H]-TdR to each well. Harvesting: 6) Wash cells 2 times with ice cold PBS. 7) Wash cells 2 times with 5% TCA.
3H-Thymidine Uptake by Cultured Cells
Materials Fibroblast cells in log phase growth Ca , Mg free-phosphate buffered saline (PBSA) 5% (w/v) Glutaraldehyde (GTA) 2% (w/v) Perchloric Acid (PCA) Subbed slides (coated with chrom alum gelatin
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