The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using NELFE antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using NELFE antibody.
Immunohistochemistry of paraffin-embedded human colon carcinoma using NELFE antibody.
Immunohistochemistry of paraffin-embedded mouse Intestine using NELFE antibody.
Immunohistochemistry of paraffin-embedded mouse liver using NELFE antibody.
Immunofluorescence analysis of A-549 cells using NELFE antibody.
Background:
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.