The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:100
IF
1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PIK3CA antibody.
Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody.
Immunohistochemistry of paraffin-embedded human prostate using PIK3CA antibody.
Immunohistochemistry of
paraffin-embedded human gastric cancer
using PIK3CA antibody.
Immunohistochemistry of
paraffin-embedded mouse brain using
PIK3CA antibody.
Background:
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.