The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:100
IF
1:50 - 1:100
Western blot analysis of extracts of HL-60 cells, using BCL2 antibody
Western blot analysis of extracts of
various cells, using BCL2 antibody
Immunohistochemistry of paraffin-embedded
human lung cancer using BCL2 antibody
Immunohistochemistry of paraffin-embedded rat brain using BCL2 antibody
Immunohistochemistry of paraffin-embedded mouse testis using BCL2 antibody
Immunofluorescence analysis of C6 cells using BCL2 Polyclonal Antibody
Immunofluorescence analysis of
NIH-3T3 cells using BCL2 Polyclonal
Antibody
Immunofluorescence analysis of U-2 OS
cells using BCL2 Polyclonal Antibody
Background:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.