The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:1000 - 1:3000
IHC
1:50 - 1:500
Western blot analysis of extracts of various cell lines, using FKRP antibody.
Background:
This gene encodes a protein which is targeted to the medial Golgi apparatus and is necessary for posttranslational modification of dystroglycan. Mutations in this gene have been associated with congenital muscular dystrophy, mental retardation, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.