The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:1000 - 1:2000
IHC
1:50 - 1:500
Western blot analysis of extracts of K561 cells, using CD40LG antibody.
Background:
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.