pBHGlox(delta)E13Cre腺病毒包装质粒

基本信息
名称：pBHGlox(delta)E13Cre腺病毒包装质粒
别称:pBHGloxdelE13cre;Adm-ax

原核抗性:	Amp
克隆菌株:	Stbl3
培养条件:	30度

质粒属性

载体宿主：	哺乳细胞,腺病毒
载体用途：	包装辅助
基因种属：
基因类型：	Cre
原核抗性：	Amp
真核抗性：
荧光蛋白：

质粒简介
  pBHGlox(delta)E1,3Cre是一个腺病毒包装质粒。

质粒图谱


质粒序列
LOCUS       Exported               34707 bp ds-DNA     circular SYN 04-NOV-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pBHGlox(delta)E1,3Cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 34707)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..34707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    559..693
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    712..760
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1242..2270)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /note="Cre"
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARD-MARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(2271..2291)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     promoter        complement(2424..2627)
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2857..2961
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2962..3822
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3993..4581
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4901..4934
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8473..8576
                     /note="Ad SA"
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(15669..15680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
ORIGIN
        1 ttattttgga ttgaagccaa tatgataatg agggggtgga gtttgtgacg tggcgcgggg
       61 cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg
      121 gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt tggtgtgcgc cggatccaca
      181 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
      241 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
      301 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
      361 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      421 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
      481 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgttc gaaatttggg
      541 ggatcttcga tgctagacga tccagacatg ataagataca ttgatgagtt tggacaaacc
      601 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      661 tttgtaacca ttataagctg caataaacaa gttgctcgaa gtcgacgatc cgaacaaacg
      721 ncccaacacc cgtgcgtttt attctgtctt tttattgccg atcccctcag aagaactcgt
      781 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
      841 ggaagcggnc agcccattcg ccgccatgtt tttnagcaat atcacgggta gccaacgcta
      901 tgtcctgata gcggtccgcc acacccagcc tcgacaattc caaccttacc caagagttcg
      961 ccaaactcag acatcacttt agcaaaaccg cgccgtgctt cttcctcggt ggcattcatc
     1021 acgaaatgtt cagcactacg catacttttg gacaggaaac gcaacggata ttgagtcaat
     1081 atcaggcatt ctatcgctca gctttacagt gacaatgncg gctggcgact gaatattagt
     1141 gcttacagac agcactacat attttccgtc gatgttgaaa tcctttctca tatgtcncca
     1201 taaatatcaa ataattatag caatcattta cgcgttaatg gctaatcgcc atcttccagc
     1261 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt
     1321 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata
     1381 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag
     1441 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga
     1501 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac
     1561 aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta
     1621 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg
     1681 ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg
     1741 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt
     1801 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt
     1861 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc
     1921 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga
     1981 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
     2041 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
     2101 aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca
     2161 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
     2221 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga caccttcctc
     2281 ttcttcttgg gcatggtcga gtctagactc gagggatcct cgactcgaag actgatcccc
     2341 aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
     2401 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
     2461 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     2521 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     2581 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     2641 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     2701 taatgccagg gaattagatc cactagacga tgataagctg tcaaacatga gaattcttga
     2761 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2821 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2881 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2941 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3001 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3061 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3121 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3181 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     3241 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3301 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3361 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3421 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3481 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     3541 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3601 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3661 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3721 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3781 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3841 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3901 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3961 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4141 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4201 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4261 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4321 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4381 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4441 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4501 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4561 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4621 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4681 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4741 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4801 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4861 agccagtaat cgaattcaag cttgtcgact cgaagatcca ataacttcgt atagcataca
     4921 ttatacgaag ttataagtac tgaattcgga tctgggcgtg gttaagggtg ggaaagaata
     4981 tataaggtgg gggtcttatg tagttttgta tctgttttgc agcagccgcc gccgccatga
     5041 gcaccaactc gtttgatgga agcattgtga gctcatattt gacaacgcgc atgcccccat
     5101 gggccggggt gcgtcagaat gtgatgggct ccagcattga tggtcgcccc gtcctgcccg
     5161 caaactctac taccttgacc tacgagaccg tgtctggaac gccgttggag actgcagcct
     5221 ccgccgccgc ttcagccgct gcagccaccg cccgcgggat tgtgactgac tttgctttcc
     5281 tgagcccgct tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac aagttgacgg
     5341 ctcttttggc acaattggat tctttgaccc gggaacttaa tgtcgtttct cagcagctgt
     5401 tggatctgcg ccagcaggtt tctgccctga aggcttcctc ccctcccaat gcggtttaaa
     5461 acataaataa aaaaccagac tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt
     5521 atttaggggt tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc
     5581 tgtgtatttt ttccaggacg tggtaaaggt gactctggat gttcagatac atgggcataa
     5641 gcccgtctct ggggtggagg tagcaccact gcagagcttc atgctgcggg gtggtgttgt
     5701 agatgatcca gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca
     5761 agctgattgc caggggcagg cccttggtgt aagtgtttac aaagcggtta agctgggatg
     5821 ggtgcatacg tggggatatg agatgcatct tggactgtat ttttaggttg gctatgttcc
     5881 cagccatatc cctccgggga ttcatgttgt gcagaaccac cagcacagtg tatccggtgc
     5941 acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg gagacgccct
     6001 tgtgacctcc aagattttcc atgcattcgt ccataatgat ggcaatgggc ccacgggcgg
     6061 cggcctgggc gaagatattt ctgggatcac taacgtcata gttgtgttcc aggatgagat
     6121 cgtcataggc catttttaca aagcgcgggc ggagggtgcc agactgcggt ataatggttc
     6181 catccggccc aggggcgtag ttaccctcac agatttgcat ttcccacgct ttgagttcag
     6241 atggggggat catgtctacc tgcggggcga tgaagaaaac ggtttccggg gtaggggaga
     6301 tcagctggga agaaagcagg ttcctgagca gctgcgactt accgcagccg gtgggcccgt
     6361 aaatcacacc tattaccggg tgcaactggt agttaagaga gctgcagctg ccgtcatccc
     6421 tgagcagggg ggccacttcg ttaagcatgt ccctgactcg catgttttcc ctgaccaaat
     6481 ccgccagaag gcgctcgccg cccagcgata gcagttcttg caaggaagca aagtttttca
     6541 acggtttgag accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc
     6601 ggtcccacag ctcggtcacc tgctctacgg catctcgatc cagcatatct cctcgtttcg
     6661 cgggttgggg cggctttcgc tgtacggcag tagtcggtgc tcgtccagac gggccagggt
     6721 catgtctttc cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg
     6781 cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg
     6841 ctgccggtct tcgccctgcg cgtcggccag gtagcatttg accatggtgt catagtccag
     6901 cccctccgcg gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg
     6961 gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga aataccgatt ccggggagta
     7021 ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc acgagccagg tgagctctgg
     7081 ccgttcgggg tcaaaaacca ggtttccccc atgctttttg atgcgtttct tacctctggt
     7141 ttccatgagc cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga
     7201 cttgagaggc ctgtcctcga gcggtgttcc gcggtcctcc tcgtatagaa actcggacca
     7261 ctctgagaca aaggctcgcg tccaggccag cacgaaggag gctaagtggg aggggtagcg
     7321 gtcgttgtcc actagggggt ccactcgctc cagggtgtga agacacatgt cgccctcttc
     7381 ggcatcaagg aaggtgattg gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg
     7441 ggggctataa aagggggtgg gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc
     7501 gagggccagc tgttggggtg agtactccct ctgaaaagcg ggcatgactt ctgcgctaag
     7561 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt
     7621 gagggtggcc gcatccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc
     7681 aaacgacccg tagagggcgt tggacagcaa cttggcgatg gagcgcaggg tttggttttt
     7741 gtcgcgatcg gcgcgctcct tggccgcgat gtttagctgc acgtattcgc gcgcaacgca
     7801 ccgccattcg ggaaagacgg tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg
     7861 gttgtgcagg gtgacaaggt caacgctggt ggctacctct ccgcgtaggc gctcgttggt
     7921 ccagcagagg cggccgccct tgcgcgagca gaatggcggt agggggtcta gctgcgtctc
     7981 gtccgggggg tctgcgtcca cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc
     8041 tatcttgcat ccttgcaagt ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc
     8101 gtatgggttg agtgggggac cccatggcat ggggtgggtg agcgcggagg cgtacatgcc
     8161 gcaaatgtcg taaacgtaga ggggctctct gagtattcca agatatgtag ggtagcatct
     8221 tccaccgcgg atgctggcgc gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc
     8281 gggaccgagg ttgctacggg cgggctgctc tgctcggaag actatctgcc tgaagatggc
     8341 atgtgagttg gatgatatgg ttggacgctg gaagacgttg aagctggcgt ctgtgagacc
     8401 taccgcgtca cgcacgaagg aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt
     8461 gacctgcacg tctagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
     8521 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtactc
     8581 ttggatcgga aacccgtcgg cctccgaacg gtaagagcct agcatgtaga actggttgac
     8641 ggcctggtag gcgcagcatc ccttttctac gggtagcgcg tatgcctgcg cggccttccg
     8701 gagcgaggtg tgggtgagcg caaaggtgtc cctgaccatg actttgaggt actggtattt
     8761 gaagtcagtg tcgtcgcatc cgccctgctc ccagagcaaa aagtccgtgc gctttttgga
     8821 acgcggattt ggcagggcga aggtgacatc gttgaagagt atctttcccg cgcgaggcat
     8881 aaagttgcgt gtgatgcgga agggtcccgg cacctcggaa cggttgttaa ttacctgggc
     8941 ggcgagcacg atctcgtcaa agccgttgat gttgtggccc acaatgtaaa gttccaagaa
     9001 gcgcgggatg cccttgatgg aaggcaattt tttaagttcc tcgtaggtga gctcttcagg
     9061 ggagctgagc ccgtgctctg aaagggccca gtctgcaaga tgagggttgg aagcgacgaa
     9121 tgagctccac aggtcacggg ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg
     9181 gcgacctatg gccatttttt ctggggtgat gcagtagaag gtaagcgggt cttgttccca
     9241 gcggtcccat ccaaggttcg cggctaggtc tcgcgcggca gtcactagag gctcatctcc
     9301 gccgaacttc atgaccagca tgaagggcac gagctgcttc ccaaaggccc ccatccaagt
     9361 ataggtctct acatcgtagg tgacaaagag acgctcggtg cgaggatgcg agccgatcgg
     9421 gaagaactgg atctcccgcc accaattgga ggagtggcta ttgatgtggt gaaagtagaa
     9481 gtccctgcga cgggccgaac actcgtgctg gcttttgtaa aaacgtgcgc agtactggca
     9541 gcggtgcacg ggctgtacat cctgcacgag gttgacctga cgaccgcgca caaggaagca
     9601 gagtgggaat ttgagcccct cgcctggcgg gtttggctgg tggtcttcta cttcggctgc
     9661 ttgtccttga ccgtctggct gctcgagggg agttacggtg gatcggacca ccacgccgcg
     9721 cgagcccaaa gtccagatgt ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag
     9781 atgggagctg tccatggtct ggagctcccg cggcgtcagg tcaggcggga gctcctgcag
     9841 gtttacctcg catagacggg tcagggcgcg ggctagatcc aggtgatacc taatttccag
     9901 gggctggttg gtggcggcgt cgatggcttg caagaggccg catccccgcg gcgcgactac
     9961 ggtaccgcgc ggcgggcggt gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg
    10021 tgacgcgggc gagcccccgg aggtaggggg ggctccggac ccgccgggag agggggcagg
    10081 ggcacgtcgg cgccgcgcgc gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac
    10141 gcgacgacgc ggcggttgat ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg
    10201 gtgagcttga gcctgaaaga gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc
    10261 tggcgcaaaa tctcctgcac gtctcctgag ttgtcttgat aggcgatctc ggccatgaac
    10321 tgctcgatct cttcctcctg gagatctccg cgtccggctc gctccacggt ggcggcgagg
    10381 tcgttggaaa tgcgggccat gagctgcgag aaggcgttga ggcctccctc gttccagacg
    10441 cggctgtaga ccacgccccc ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg
    10501 agctccacgt gccgggcgaa gacggcgtag tttcgcaggc gctgaaagag gtagttgagg
    10561 gtggtggcgg tgtgttctgc cacgaagaag tacataaccc agcgtcgcaa cgtggattcg
    10621 ttgatatccc ccaaggcctc aaggcgctcc atggcctcgt agaagtccac ggcgaagttg
    10681 aaaaactggg agttgcgcgc cgacacggtt aactcctcct ccagaagacg gatgagctcg
    10741 gcgacagtgt cgcgcacctc gcgctcaaag gctacagggg cctcttcttc ttcttcaatc
    10801 tcctcttcca taagggcctc cccttcttct tcttctggcg gcggtggggg aggggggaca
    10861 cggcggcgac gacggcgcac cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg
    10921 cgacggcgca tggtctcggt gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg
    10981 ccgcccgtca tgtcccggtt atgggttggc ggggggctgc catgcggcag ggatacggcg
    11041 ctaacgatgc atctcaacaa ttgttgtgta ggtactccgc cgccgaggga cctgagcgag
    11101 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
    11161 ggtaggctga gcaccgtggc gggcggcagc gggcggcggt cggggttgtt tctggcggag
    11221 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgacagaagc
    11281 accatgtcct tgggtccggc ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg
    11341 ttttgacatc ggcgcaggtc tttgtagtag tcttgcatga gcctttctac cggcacttct
    11401 tcttctcctt cctcttgtcc tgcatctctt gcatctatcg ctgcggcggc ggcggagttt
    11461 ggccgtaggt ggcgccctct tcctcccatg cgtgtgaccc cgaagcccct catcggctga
    11521 agcagggcta ggtcggcgac aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg
    11581 gtagactgga agtcatccat gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa
    11641 gtgcagttgg ccataacgga ccagttaacg gtctggtgac ccggctgcga gagctcggtg
    11701 tacctgagac gcgagtaagc cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg
    11761 tactggtatc ccaccaaaaa gtgcggcggc ggctggcggt agaggggcca gcgtagggtg
    11821 gccggggctc cgggggcgag atcttccaac ataaggcgat gatatccgta gatgtacctg
    11881 gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc
    11941 cagatgttgc gcagcggcaa aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc
    12001 gcgcaatcgt tgacgctcta ccgtgcaaaa ggagagcctg taagcgggca ctcttccgtg
    12061 gtctggtgga taaattcgca agggtatcat ggcggacgac cggggttcga gccccgtatc
    12121 cggccgtccg ccgtgatcca tgcggttacc gcccgcgtgt cgaacccagg tgtgcgacgt
    12181 cagacaacgg gggagtgctc cttttggctt ccttccaggc gcggcggctg ctgcgctagc
    12241 ttttttggcc actggccgcg cgcagcgtaa gcggttaggc tggaaagcga aagcattaag
    12301 tggctcgctc cctgtagccg gagggttatt ttccaagggt tgagtcgcgg gacccccggt
    12361 tcgagtctcg gaccggccgg actgcggcga acgggggttt gcctccccgt catgcaagac
    12421 cccgcttgca aattcctccg gaaacaggga cgagcccctt ttttgctttt cccagatgca
    12481 tccggtgctg cggcagatgc gcccccctcc tcagcagcgg caagagcaag agcagcggca
    12541 gacatgcagg gcaccctccc ctcctcctac cgcgtcagga ggggcgacat ccgcggttga
    12601 cgcggcagca gatggtgatt acgaaccccc gcggcgccgg gcccggcact acctggactt
    12661 ggaggagggc gagggcctgg cgcggctagg agcgccctct cctgagcggt acccaagggt
    12721 gcagctgaag cgtgatacgc gtgaggcgta cgtgccgcgg cagaacctgt ttcgcgaccg
    12781 cgagggagag gagcccgagg agatgcggga tcgaaagttc cacgcagggc gcgagctgcg
    12841 gcatggcctg aatcgcgagc ggttgctgcg cgaggaggac tttgagcccg acgcgcgaac
    12901 cgggattagt cccgcgcgcg cacacgtggc ggccgccgac ctggtaaccg catacgagca
    12961 gacggtgaac caggagatta actttcaaaa aagctttaac aaccacgtgc gtacgcttgt
    13021 ggcgcgcgag gaggtggcta taggactgat gcatctgtgg gactttgtaa gcgcgctgga
    13081 gcaaaaccca aatagcaagc cgctcatggc gcagctgttc cttatagtgc agcacagcag
    13141 ggacaacgag gcattcaggg atgcgctgct aaacatagta gagcccgagg gccgctggct
    13201 gctcgatttg ataaacatcc tgcagagcat agtggtgcag gagcgcagct tgagcctggc
    13261 tgacaaggtg gccgccatca actattccat gcttagcctg ggcaagtttt acgcccgcaa
    13321 gatataccat accccttacg ttcccataga caaggaggta aagatcgagg ggttctacat
    13381 gcgcatggcg ctgaaggtgc ttaccttgag cgacgacctg ggcgtttatc gcaacgagcg
    13441 catccacaag gccgtgagcg tgagccggcg gcgcgagctc agcgaccgcg agctgatgca
    13501 cagcctgcaa agggccctgg ctggcacggg cagcggcgat agagaggccg agtcctactt
    13561 tgacgcgggc gctgacctgc gctgggcccc aagccgacgc gccctggagg cagctggggc
    13621 cggacctggg ctggcggtgg cacccgcgcg cgctggcaac gtcggcggcg tggaggaata
    13681 tgacgaggac gatgagtacg agccagagga cggcgagtac taagcggtga tgtttctgat
    13741 cagatgatgc aagacgcaac ggacccggcg gtgcgggcgg cgctgcagag ccagccgtcc
    13801 ggccttaact ccacggacga ctggcgccag gtcatggacc gcatcatgtc gctgactgcg
    13861 cgcaatcctg acgcgttccg gcagcagccg caggccaacc ggctctccgc aattctggaa
    13921 gcggtggtcc cggcgcgcgc aaaccccacg cacgagaagg tgctggcgat cgtaaacgcg
    13981 ctggccgaaa acagggccat ccggcccgac gaggccggcc tggtctacga cgcgctgctt
    14041 cagcgcgtgg ctcgttacaa cagcggcaac gtgcagacca acctggaccg gctggtgggg
    14101 gatgtgcgcg aggccgtggc gcagcgtgag cgcgcgcagc agcagggcaa cctgggctcc
    14161 atggttgcac taaacgcctt cctgagtaca cagcccgcca acgtgccgcg gggacaggag
    14221 gactacacca actttgtgag cgcactgcgg ctaatggtga ctgagacacc gcaaagtgag
    14281 gtgtaccagt ctgggccaga ctattttttc cagaccagta gacaaggcct gcagaccgta
    14341 aacctgagcc aggctttcaa aaacttgcag gggctgtggg gggtgcgggc tcccacaggc
    14401 gaccgcgcga ccgtgtctag cttgctgacg cccaactcgc gcctgttgct gctgctaata
    14461 gcgcccttca cggacagtgg cagcgtgtcc cgggacacat acctaggtca cttgctgaca
    14521 ctgtaccgcg aggccatagg tcaggcgcat gtggacgagc atactttcca ggagattaca
    14581 agtgtcagcc gcgcgctggg gcaggaggac acgggcagcc tggaggcaac cctaaactac
    14641 ctgctgacca accggcggca gaagatcccc tcgttgcaca gtttaaacag cgaggaggag
    14701 cgcattttgc gctacgtgca gcagagcgtg agccttaacc tgatgcgcga cggggtaacg
    14761 cccagcgtgg cgctggacat gaccgcgcgc aacatggaac cgggcatgta tgcctcaaac
    14821 cggccgttta tcaaccgcct aatggactac ttgcatcgcg cggccgccgt gaaccccgag
    14881 tatttcacca atgccatctt gaacccgcac tggctaccgc cccctggttt ctacaccggg
    14941 ggattcgagg tgcccgaggg taacgatgga ttcctctggg acgacataga cgacagcgtg
    15001 ttttccccgc aaccgcagac cctgctagag ttgcaacagc gcgagcaggc agaggcggcg
    15061 ctgcgaaagg aaagcttccg caggccaagc agcttgtccg atctaggcgc tgcggccccg
    15121 cggtcagatg ctagtagccc atttccaagc ttgatagggt ctcttaccag cactcgcacc
    15181 acccgcccgc gcctgctggg cgaggaggag tacctaaaca actcgctgct gcagccgcag
    15241 cgcgaaaaaa acctgcctcc ggcatttccc aacaacggga tagagagcct agtggacaag
    15301 atgagtagat ggaagacgta cgcgcaggag cacagggacg tgccaggccc gcgcccgccc
    15361 acccgtcgtc aaaggcacga ccgtcagcgg ggtctggtgt gggaggacga tgactcggca
    15421 gacgacagca gcgtcctgga tttgggaggg agtggcaacc cgtttgcgca ccttcgcccc
    15481 aggctgggga gaatgtttta aaaaaaaaaa agcatgatgc aaaataaaaa actcaccaag
    15541 gccatggcac cgagcgttgg ttttcttgta ttccccttag tatgcggcgc gcggcgatgt
    15601 atgaggaagg tcctcctccc tcctacgaga gtgtggtgag cgcggcgcca gtggcggcgg
    15661 cgctgggttc tcccttcgat gctcccctgg acccgccgtt tgtgcctccg cggtacctgc
    15721 ggcctaccgg ggggagaaac agcatccgtt actctgagtt ggcaccccta ttcgacacca
    15781 cccgtgtgta cctggtggac aacaagtcaa cggatgtggc atccctgaac taccagaacg
    15841 accacagcaa ctttctgacc acggtcattc aaaacaatga ctacagcccg ggggaggcaa
    15901 gcacacagac catcaatctt gacgaccggt cgcactgggg cggcgacctg aaaaccatcc
    15961 tgcataccaa catgccaaat gtgaacgagt tcatgtttac caataagttt aaggcgcggg
    16021 tgatggtgtc gcgcttgcct actaaggaca atcaggtgga gctgaaatac gagtgggtgg
    16081 agttcacgct gcccgagggc aactactccg agaccatgac catagacctt atgaacaacg
    16141 cgatcgtgga gcactacttg aaagtgggca gacagaacgg ggttctggaa agcgacatcg
    16201 gggtaaagtt tgacacccgc aacttcagac tggggtttga ccccgtcact ggtcttgtca
    16261 tgcctggggt atatacaaac gaagccttcc atccagacat cattttgctg ccaggatgcg
    16321 gggtggactt cacccacagc cgcctgagca acttgttggg catccgcaag cggcaaccct
    16381 tccaggaggg ctttaggatc acctacgatg atctggaggg tggtaacatt cccgcactgt
    16441 tggatgtgga cgcctaccag gcgagcttga aagatgacac cgaacagggc gggggtggcg
    16501 caggcggcag caacagcagt ggcagcggcg cggaagagaa ctccaacgcg gcagccgcgg
    16561 caatgcagcc ggtggaggac atgaacgatc atgccattcg cggcgacacc tttgccacac
    16621 gggctgagga gaagcgcgct gaggccgaag cagcggccga agctgccgcc cccgctgcgc
    16681 aacccgaggt cgagaagcct cagaagaaac cggtgatcaa acccctgaca gaggacagca
    16741 agaaacgcag ttacaaccta ataagcaatg acagcacctt cacccagtac cgcagctggt
    16801 accttgcata caactacggc gaccctcaga ccggaatccg ctcatggacc ctgctttgca
    16861 ctcctgacgt aacctgcggc tcggagcagg tctactggtc gttgccagac atgatgcaag
    16921 accccgtgac cttccgctcc acgcgccaga tcagcaactt tccggtggtg ggcgccgagc
    16981 tgttgcccgt gcactccaag agcttctaca acgaccaggc cgtctactcc caactcatcc
    17041 gccagtttac ctctctgacc cacgtgttca atcgctttcc cgagaaccag attttggcgc
    17101 gcccgccagc ccccaccatc accaccgtca gtgaaaacgt tcctgctctc acagatcacg
    17161 ggacgctacc gctgcgcaac agcatcggag gagtccagcg agtgaccatt actgacgcca
    17221 gacgccgcac ctgcccctac gtttacaagg ccctgggcat agtctcgccg cgcgtcctat
    17281 cgagccgcac tttttgagca agcatgtcca tccttatatc gcccagcaat aacacaggct
    17341 ggggcctgcg cttcccaagc aagatgtttg gcggggccaa gaagcgctcc gaccaacacc
    17401 cagtgcgcgt gcgcgggcac taccgcgcgc cctggggcgc gcacaaacgc ggccgcactg
    17461 ggcgcaccac cgtcgatgac gccatcgacg cggtggtgga ggaggcgcgc aactacacgc
    17521 ccacgccgcc accagtgtcc acagtggacg cggccattca gaccgtggtg cgcggagccc
    17581 ggcgctatgc taaaatgaag agacggcgga ggcgcgtagc acgtcgccac cgccgccgac
    17641 ccggcactgc cgcccaacgc gcggcggcgg ccctgcttaa ccgcgcacgt cgcaccggcc
    17701 gacgggcggc catgcgggcc gctcgaaggc tggccgcggg tattgtcact gtgcccccca
    17761 ggtccaggcg acgagcggcc gccgcagcag ccgcggccat tagtgctatg actcagggtc
    17821 gcaggggcaa cgtgtattgg gtgcgcgact cggttagcgg cctgcgcgtg cccgtgcgca
    17881 cccgcccccc gcgcaactag attgcaagaa aaaactactt agactcgtac tgttgtatgt
    17941 atccagcggc ggcggcgcgc aacgaagcta tgtccaagcg caaaatcaaa gaagagatgc
    18001 tccaggtcat cgcgccggag atctatggcc ccccgaagaa ggaagagcag gattacaagc
    18061 cccgaaagct aaagcgggtc aaaaagaaaa agaaagatga tgatgatgaa cttgacgacg
    18121 aggtggaact gctgcacgct accgcgccca ggcgacgggt acagtggaaa ggtcgacgcg
    18181 taaaacgtgt tttgcgaccc ggcaccaccg tagtctttac gcccggtgag cgctccaccc
    18241 gcacctacaa gcgcgtgtat gatgaggtgt acggcgacga ggacctgctt gagcaggcca
    18301 acgagcgcct cggggagttt gcctacggaa agcggcataa ggacatgctg gcgttgccgc
    18361 tggacgaggg caacccaaca cctagcctaa agcccgtaac actgcagcag gtgctgcccg
    18421 cgcttgcacc gtccgaagaa aagcgcggcc taaagcgcga gtctggtgac ttggcaccca
    18481 ccgtgcagct gatggtaccc aagcgccagc gactggaaga tgtcttggaa aaaatgaccg
    18541 tggaacctgg gctggagccc gaggtccgcg tgcggccaat caagcaggtg gcgccgggac
    18601 tgggcgtgca gaccgtggac gttcagatac ccactaccag tagcaccagt attgccaccg
    18661 ccacagaggg catggagaca caaacgtccc cggttgcctc agcggtggcg gatgccgcgg
    18721 tgcaggcggt cgctgcggcc gcgtccaaga cctctacgga ggtgcaaacg gacccgtgga
    18781 tgtttcgcgt ttcagccccc cggcgcccgc gcggttcgag gaagtacggc gccgccagcg
    18841 cgctactgcc cgaatatgcc ctacatcctt ccattgcgcc tacccccggc tatcgtggct
    18901 acacctaccg ccccagaaga cgagcaacta cccgacgccg aaccaccact ggaacccgcc
    18961 gccgccgtcg ccgtcgccag cccgtgctgg ccccgatttc cgtgcgcagg gtggctcgcg
    19021 aaggaggcag gaccctggtg ctgccaacag cgcgctacca ccccagcatc gtttaaaagc
    19081 cggtctttgt ggttcttgca gatatggccc tcacctgccg cctccgtttc ccggtgccgg
    19141 gattccgagg aagaatgcac cgtaggaggg gcatggccgg ccacggcctg acgggcggca
    19201 tgcgtcgtgc gcaccaccgg cggcggcgcg cgtcgcaccg tcgcatgcgc ggcggtatcc
    19261 tgcccctcct tattccactg atcgccgcgg cgattggcgc cgtgcccgga attgcatccg
    19321 tggccttgca ggcgcagaga cactgattaa aaacaagttg catgtggaaa aatcaaaata
    19381 aaaagtctgg actctcacgc tcgcttggtc ctgtaactat tttgtagaat ggaagacatc
    19441 aactttgcgt ctctggcccc gcgacacggc tcgcgcccgt tcatgggaaa ctggcaagat
    19501 atcggcacca gcaatatgag cggtggcgcc ttcagctggg gctcgctgtg gagcggcatt
    19561 aaaaatttcg gttccaccgt taagaactat ggcagcaagg cctggaacag cagcacaggc
    19621 cagatgctga gggataagtt gaaagagcaa aatttccaac aaaaggtggt agatggcctg
    19681 gcctctggca ttagcggggt ggtggacctg gccaaccagg cagtgcaaaa taagattaac
    19741 agtaagcttg atccccgccc tcccgtagag gagcctccac cggccgtgga gacagtgtct
    19801 ccagaggggc gtggcgaaaa gcgtccgcgc cccgacaggg aagaaactct ggtgacgcaa
    19861 atagacgagc ctccctcgta cgaggaggca ctaaagcaag gcctgcccac cacccgtccc
    19921 atcgcgccca tggctaccgg agtgctgggc cagcacacac ccgtaacgct ggacctgcct
    19981 ccccccgccg acacccagca gaaacctgtg ctgccaggcc cgaccgccgt tgttgtaacc
    20041 cgtcctagcc gcgcgtccct gcgccgcgcc gccagcggtc cgcgatcgtt gcggcccgta
    20101 gccagtggca actggcaaag cacactgaac agcatcgtgg gtctgggggt gcaatccctg
    20161 aagcgccgac gatgcttctg aatagctaac gtgtcgtatg tgtgtcatgt atgcgtccat
    20221 gtcgccgcca gaggagctgc tgagccgccg cgcgcccgct ttccaagatg gctacccctt
    20281 cgatgatgcc gcagtggtct tacatgcaca tctcgggcca ggacgcctcg gagtacctga
    20341 gccccgggct ggtgcagttt gcccgcgcca ccgagacgta cttcagcctg aataacaagt
    20401 ttagaaaccc cacggtggcg cctacgcacg acgtgaccac agaccggtcc cagcgtttga
    20461 cgctgcggtt catccctgtg gaccgtgagg atactgcgta ctcgtacaag gcgcggttca
    20521 ccctagctgt gggtgataac cgtgtgctgg acatggcttc cacgtacttt gacatccgcg
    20581 gcgtgctgga caggggccct acttttaagc cctactctgg cactgcctac aacgccctgg
    20641 ctcccaaggg tgccccaaat ccttgcgaat gggatgaagc tgctactgct cttgaaataa
    20701 acctagaaga agaggacgat gacaacgaag acgaagtaga cgagcaagct gagcagcaaa
    20761 aaactcacgt atttgggcag gcgccttatt ctggtataaa tattacaaag gagggtattc
    20821 aaataggtgt cgaaggtcaa acacctaaat atgccgataa aacatttcaa cctgaacctc
    20881 aaataggaga atctcagtgg tacgaaactg aaattaatca tgcagctggg agagtcctta
    20941 aaaagactac cccaatgaaa ccatgttacg gttcatatgc aaaacccaca aatgaaaatg
    21001 gagggcaagg cattcttgta aagcaacaaa atggaaagct agaaagtcaa gtggaaatgc
    21061 aatttttctc aactactgag gcgaccgcag gcaatggtga taacttgact cctaaagtgg
    21121 tattgtacag tgaagatgta gatatagaaa ccccagacac tcatatttct tacatgccca
    21181 ctattaagga aggtaactca cgagaactaa tgggccaaca atctatgccc aacaggccta
    21241 attacattgc ttttagggac aattttattg gtctaatgta ttacaacagc acgggtaata
    21301 tgggtgttct ggcgggccaa gcatcgcagt tgaatgctgt tgtagatttg caagacagaa
    21361 acacagagct ttcataccag cttttgcttg attccattgg tgatagaacc aggtactttt
    21421 ctatgtggaa tcaggctgtt gacagctatg atccagatgt tagaattatt gaaaatcatg
    21481 gaactgaaga tgaacttcca aattactgct ttccactggg aggtgtgatt aatacagaga
    21541 ctcttaccaa ggtaaaacct aaaacaggtc aggaaaatgg atgggaaaaa gatgctacag
    21601 aattttcaga taaaaatgaa ataagagttg gaaataattt tgccatggaa atcaatctaa
    21661 atgccaacct gtggagaaat ttcctgtact ccaacatagc gctgtatttg cccgacaagc
    21721 taaagtacag tccttccaac gtaaaaattt ctgataaccc aaacacctac gactacatga
    21781 acaagcgagt ggtggctccc gggttagtgg actgctacat taaccttgga gcacgctggt
    21841 cccttgacta tatggacaac gtcaacccat ttaaccacca ccgcaatgct ggcctgcgct
    21901 accgctcaat gttgctgggc aatggtcgct atgtgccctt ccacatccag gtgcctcaga
    21961 agttctttgc cattaaaaac ctccttctcc tgccgggctc atacacctac gagtggaact
    22021 tcaggaagga tgttaacatg gttctgcaga gctccctagg aaatgaccta agggttgacg
    22081 gagccagcat taagtttgat agcatttgcc tttacgccac cttcttcccc atggcccaca
    22141 acaccgcctc cacgcttgag gccatgctta gaaacgacac caacgaccag tcctttaacg
    22201 actatctctc cgccgccaac atgctctacc ctatacccgc caacgctacc aacgtgccca
    22261 tatccatccc ctcccgcaac tgggcggctt tccgcggctg ggccttcacg cgccttaaga
    22321 ctaaggaaac cccatcactg ggctcgggct acgaccctta ttacacctac tctggctcta
    22381 taccctacct agatggaacc ttttacctca accacacctt taagaaggtg gccattacct
    22441 ttgactcttc tgtcagctgg cctggcaatg accgcctgct tacccccaac gagtttgaaa
    22501 ttaagcgctc agttgacggg gagggttaca acgttgccca gtgtaacatg accaaagact
    22561 ggttcctggt acaaatgcta gctaactaca acattggcta ccagggcttc tatatcccag
    22621 agagctacaa ggaccgcatg tactccttct ttagaaactt ccagcccatg agccgtcagg
    22681 tggtggatga tactaaatac aaggactacc aacaggtggg catcctacac caacacaaca
    22741 actctggatt tgttggctac cttgccccca ccatgcgcga aggacaggcc taccctgcta
    22801 acttccccta tccgcttata ggcaagaccg cagttgacag cattacccag aaaaagtttc
    22861 tttgcgatcg caccctttgg cgcatcccat tctccagtaa ctttatgtcc atgggcgcac
    22921 tcacagacct gggccaaaac cttctctacg ccaactccgc ccacgcgcta gacatgactt
    22981 ttgaggtgga tcccatggac gagcccaccc ttctttatgt tttgtttgaa gtctttgacg
    23041 tggtccgtgt gcaccggccg caccgcggcg tcatcgaaac cgtgtacctg cgcacgccct
    23101 tctcggccgg caacgccaca acataaagaa gcaagcaaca tcaacaacag ctgccgccat
    23161 gggctccagt gagcaggaac tgaaagccat tgtcaaagat cttggttgtg ggccatattt
    23221 tttgggcacc tatgacaagc gctttccagg ctttgtttct ccacacaagc tcgcctgcgc
    23281 catagtcaat acggccggtc gcgagactgg gggcgtacac tggatggcct ttgcctggaa
    23341 cccgcactca aaaacatgct acctctttga gccctttggc ttttctgacc agcgactcaa
    23401 gcaggtttac cagtttgagt acgagtcact cctgcgccgt agcgccattg cttcttcccc
    23461 cgaccgctgt ataacgctgg aaaagtccac ccaaagcgta caggggccca actcggccgc
    23521 ctgtggacta ttctgctgca tgtttctcca cgcctttgcc aactggcccc aaactcccat
    23581 ggatcacaac cccaccatga accttattac cggggtaccc aactccatgc tcaacagtcc
    23641 ccaggtacag cccaccctgc gtcgcaacca ggaacagctc tacagcttcc tggagcgcca
    23701 ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt tttgtcactt
    23761 gaaaaacatg taaaaataat gtactagaga cactttcaat aaaggcaaat gcttttattt
    23821 gtacactctc gggtgattat ttacccccac ccttgccgtc tgcgccgttt aaaaatcaaa
    23881 ggggttctgc cgcgcatcgc tatgcgccac tggcagggac acgttgcgat actggtgttt
    23941 agtgctccac ttaaactcag gcacaaccat ccgcggcagc tcggtgaagt tttcactcca
    24001 caggctgcgc accatcacca acgcgtttag caggtcgggc gccgatatct tgaagtcgca
    24061 gttggggcct ccgccctgcg cgcgcgagtt gcgatacaca gggttgcagc actggaacac
    24121 tatcagcgcc gggtggtgca cgctggccag cacgctcttg tcggagatca gatccgcgtc
    24181 caggtcctcc gcgttgctca gggcgaacgg agtcaacttt ggtagctgcc ttcccaaaaa
    24241 gggcgcgtgc ccaggctttg agttgcactc gcaccgtagt ggcatcaaaa ggtgaccgtg
    24301 cccggtctgg gcgttaggat acagcgcctg cataaaagcc ttgatctgct taaaagccac
    24361 ctgagccttt gcgccttcag agaagaacat gccgcaagac ttgccggaaa actgattggc
    24421 cggacaggcc gcgtcgtgca cgcagcacct tgcgtcggtg ttggagatct gcaccacatt
    24481 tcggccccac cggttcttca cgatcttggc cttgctagac tgctccttca gcgcgcgctg
    24541 cccgttttcg ctcgtcacat ccatttcaat cacgtgctcc ttatttatca taatgcttcc
    24601 gtgtagacac ttaagctcgc cttcgatctc agcgcagcgg tgcagccaca acgcgcagcc
    24661 cgtgggctcg tgatgcttgt aggtcacctc tgcaaacgac tgcaggtacg cctgcaggaa
    24721 tcgccccatc atcgtcacaa aggtcttgtt gctggtgaag gtcagctgca acccgcggtg
    24781 ctcctcgttc agccaggtct tgcatacggc cgccagagct tccacttggt caggcagtag
    24841 tttgaagttc gcctttagat cgttatccac gtggtacttg tccatcagcg cgcgcgcagc
    24901 ctccatgccc ttctcccacg cagacacgat cggcacactc agcgggttca tcaccgtaat
    24961 ttcactttcc gcttcgctgg gctcttcctc ttcctcttgc gtccgcatac cacgcgccac
    25021 tgggtcgtct tcattcagcc gccgcactgt gcgcttacct cctttgccat gcttgattag
    25081 caccggtggg ttgctgaaac ccaccatttg tagcgccaca tcttctcttt cttcctcgct
    25141 gtccacgatt acctctggtg atggcgggcg ctcgggcttg ggagaagggc gcttcttttt
    25201 cttcttgggc gcaatggcca aatccgccgc cgaggtcgat ggccgcgggc tgggtgtgcg
    25261 cggcaccagc gcgtcttgtg atgagtcttc ctcgtcctcg gactcgatac gccgcctcat
    25321 ccgctttttt gggggcgccc ggggaggcgg cggcgacggg gacggggacg acacgtcctc
    25381 catggttggg ggacgtcgcg ccgcaccgcg tccgcgctcg ggggtggttt cgcgctgctc
    25441 ctcttcccga ctggccattt ccttctccta taggcagaaa aagatcatgg agtcagtcga
    25501 gaagaaggac agcctaaccg ccccctctga gttcgccacc accgcctcca ccgatgccgc
    25561 caacgcgcct accaccttcc ccgtcgaggc acccccgctt gaggaggagg aagtgattat
    25621 cgagcaggac ccaggttttg taagcgaaga cgacgaggac cgctcagtac caacagagga
    25681 taaaaagcaa gaccaggaca acgcagaggc aaacgaggaa caagtcgggc ggggggacga
    25741 aaggcatggc gactacctag atgtgggaga cgacgtgctg ttgaagcatc tgcagcgcca
    25801 gtgcgccatt atctgcgacg cgttgcaaga gcgcagcgat gtgcccctcg ccatagcgga
    25861 tgtcagcctt gcctacgaac gccacctatt ctcaccgcgc gtacccccca aacgccaaga
    25921 aaacggcaca tgcgagccca acccgcgcct caacttctac cccgtatttg ccgtgccaga
    25981 ggtgcttgcc acctatcaca tctttttcca aaactgcaag atacccctat cctgccgtgc
    26041 caaccgcagc cgagcggaca agcagctggc cttgcggcag ggcgctgtca tacctgatat
    26101 cgcctcgctc aacgaagtgc caaaaatctt tgagggtctt ggacgcgacg agaagcgcgc
    26161 ggcaaacgct ctgcaacagg aaaacagcga aaatgaaagt cactctggag tgttggtgga
    26221 actcgagggt gacaacgcgc gcctagccgt actaaaacgc agcatcgagg tcacccactt
    26281 tgcctacccg gcacttaacc taccccccaa ggtcatgagc acagtcatga gtgagctgat
    26341 cgtgcgccgt gcgcagcccc tggagaggga tgcaaatttg caagaacaaa cagaggaggg
    26401 cctacccgca gttggcgacg agcagctagc gcgctggctt caaacgcgcg agcctgccga
    26461 cttggaggag cgacgcaaac taatgatggc cgcagtgctc gttaccgtgg agcttgagtg
    26521 catgcagcgg ttctttgctg acccggagat gcagcgcaag ctagaggaaa cattgcacta
    26581 cacctttcga cagggctacg tacgccaggc ctgcaagatc tccaacgtgg agctctgcaa
    26641 cctggtctcc taccttggaa ttttgcacga aaaccgcctt gggcaaaacg tgcttcattc
    26701 cacgctcaag ggcgaggcgc gccgcgacta cgtccgcgac tgcgtttact tatttctatg
    26761 ctacacctgg cagacggcca tgggcgtttg gcagcagtgc ttggaggagt gcaacctcaa
    26821 ggagctgcag aaactgctaa agcaaaactt gaaggaccta tggacggcct tcaacgagcg
    26881 ctccgtggcc gcgcacctgg cggacatcat tttccccgaa cgcctgctta aaaccctgca
    26941 acagggtctg ccagacttca ccagtcaaag catgttgcag aactttagga actttatcct
    27001 agagcgctca ggaatcttgc ccgccacctg ctgtgcactt cctagcgact ttgtgcccat
    27061 taagtaccgc gaatgccctc cgccgctttg gggccactgc taccttctgc agctagccaa
    27121 ctaccttgcc taccactctg acataatgga agacgtgagc ggtgacggtc tactggagtg
    27181 tcactgtcgc tgcaacctat gcaccccgca ccgctccctg gtttgcaatt cgcagctgct
    27241 taacgaaagt caaattatcg gtacctttga gctgcagggt ccctcgcctg acgaaaagtc
    27301 cgcggctccg gggttgaaac tcactccggg gctgtggacg tcggcttacc ttcgcaaatt
    27361 tgtacctgag gactaccacg cccacgagat taggttctac gaagaccaat cccgcccgcc
    27421 aaatgcggag cttaccgcct gcgtcattac ccagggccac attcttggcc aattgcaagc
    27481 catcaacaaa gcccgccaag agtttctgct acgaaaggga cggggggttt acttggaccc
    27541 ccagtccggc gaggagctca acccaatccc cccgccgccg cagccctatc agcagcagcc
    27601 gcgggccctt gcttcccagg atggcaccca aaaagaagct gcagctgccg ccgccaccca
    27661 cggacgagga ggaatactgg gacagtcagg cagaggaggt tttggacgag gaggaggagg
    27721 acatgatgga agactgggag agcctagacg aggaagcttc cgaggtcgaa gaggtgtcag
    27781 acgaaacacc gtcaccctcg gtcgcattcc cctcgccggc gccccagaaa tcggcaaccg
    27841 gttccagcat ggctacaacc tccgctcctc aggcgccgcc ggcactgccc gttcgccgac
    27901 ccaaccgtag atgggacacc actggaacca gggccggtaa gtccaagcag ccgccgccgt
    27961 tagcccaaga gcaacaacag cgccaaggct accgctcatg gcgcgggcac aagaacgcca
    28021 tagttgcttg cttgcaagac tgtgggggca acatctcctt cgcccgccgc tttcttctct
    28081 accatcacgg cgtggccttc ccccgtaaca tcctgcatta ctaccgtcat ctctacagcc
    28141 catactgcac cggcggcagc ggcagcggca gcaacagcag cggccacaca gaagcaaagg
    28201 cgaccggata gcaagactct gacaaagccc aagaaatcca cagcggcggc agcagcagga
    28261 ggaggagcgc tgcgtctggc gcccaacgaa cccgtatcga cccgcgagct tagaaacagg
    28321 atttttccca ctctgtatgc tatatttcaa cagagcaggg gccaagaaca agagctgaaa
    28381 ataaaaaaca ggtctctgcg atccctcacc cgcagctgcc tgtatcacaa aagcgaagat
    28441 cagcttcggc gcacgctgga agacgcggag gctctcttca gtaaatactg cgcgctgact
    28501 cttaaggact agtttcgcgc cctttctcaa atttaagcgc gaaaactacg tcatctccag
    28561 cggccacacc cggcgccagc acctgtcgtc agcgccatta tgagcaagga aattcccacg
    28621 ccctacatgt ggagttacca gccacaaatg ggacttgcgg ctggagctgc ccaagactac
    28681 tcaacccgaa taaactacat gagcgcggga ccccacatga tatcccgggt caacggaatc
    28741 cgcgcccacc gaaaccgaat tctcttggaa caggcggcta ttaccaccac acctcgtaat
    28801 aaccttaatc cccgtagttg gcccgctgcc ctggtgtacc aggaaagtcc cgctcccacc
    28861 actgtggtac ttcccagaga cgcccaggcc gaagttcaga tgactaactc aggggcgcag
    28921 cttgcgggcg gctttcgtca cagggtgcgg tcgcccgggc agggtataac tcacctgaca
    28981 atcagagggc gaggtattca gctcaacgac gagtcggtga gctcctcgct tggtctccgt
    29041 ccggacggga catttcagat cggcggcgcc ggccgtcctt cattcacgcc tcgtcaggca
    29101 atcctaactc tgcagacctc gtcctctgag ccgcgctctg gaggcattgg aactctgcaa
    29161 tttattgagg agtttgtgcc atcggtctac tttaacccct tctcgggacc tcccggccac
    29221 tatccggatc aatttattcc taactttgac gcggtaaagg actcggcgga cggctacgac
    29281 tgaatgttaa gtggagaggc agagcaactg cgcctgaaac acctggtcca ctgtcgccgc
    29341 cacaagtgct ttgcccgcga ctccggtgag ttttgctact ttgaattgcc cgaggatcat
    29401 atcgagggcc cggcgcacgg cgtccggctt accgcccagg gagagcttgc ccgtagcctg
    29461 attcgggagt ttacccagcg ccccctgcta gttgagcggg acaggggacc ctgtgttctc
    29521 actgtgattt gcaactgtcc taaccttgga ttacatcaag atcctctagt taattaacta
    29581 gagtacccgg ggatcttatt ccctttaact aataaaaaaa aataataaag catcacttac
    29641 ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt gccctcctcc
    29701 cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct aaatggaatg
    29761 tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt gcagatgaag
    29821 cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac ggaaaccggt
    29881 cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt tcaagagagt
    29941 ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa tggcatgctt
    30001 gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac ctcccaaaat
    30061 gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct ggaaatatct
    30121 gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc tctaatggtc
    30181 gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga ctccaaactt
    30241 agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct gcaaacatca
    30301 ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc ccctctaact
    30361 actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca aaatggaaaa
    30421 ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac tttgaccgta
    30481 gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt tactggagcc
    30541 ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact aaggattgat
    30601 tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca aaaccaacta
    30661 aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt ggatattaac
    30721 tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct tgaggttaac
    30781 ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa tgcaggagat
    30841 gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac aaaaattggc
    30901 catggcctag aatttgattc aaacaaggct atggttccta aactaggaac tggccttagt
    30961 tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct aactttgtgg
    31021 accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc taaactcact
    31081 ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt ggctgttaaa
    31141 ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat aagatttgac
    31201 gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa ctttagaaat
    31261 ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc taacctatca
    31321 gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca agtttactta
    31381 aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac acaggaaaca
    31441 ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc tggccacaac
    31501 tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc ccaagaataa
    31561 agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa tttcaagtca
    31621 tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac cgtaccttaa
    31681 tcaaactcac agaaccctag tattcaacct gccacctccc tcccaacaca cagagtacac
    31741 agtcctttct ccccggctgg ccttaaaaag catcatatca tgggtaacag acatattctt
    31801 aggtgttata ttccacacgg tttcctgtcg agccaaacgc tcatcagtga tattaataaa
    31861 ctccccgggc agctcactta agttcatgtc gctgtccagc tgctgagcca caggctgctg
    31921 tccaacttgc ggttgcttaa cgggcggcga aggagaagtc cacgcctaca tgggggtaga
    31981 gtcataatcg tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg
    32041 ccgccgccgc tccgtcctgc aggaatacaa catggcagtg gtctcctcag cgatgattcg
    32101 caccgcccgc agcataaggc gccttgtcct ccgggcacag cagcgcaccc tgatctcact
    32161 taaatcagca cagtaactgc agcacagcac cacaatattg ttcaaaatcc cacagtgcaa
    32221 ggcgctgtat ccaaagctca tggcggggac cacagaaccc acgtggccat cataccacaa
    32281 gcgcaggtag attaagtggc gacccctcat aaacacgctg gacataaaca ttacctcttt
    32341 tggcatgttg taattcacca cctcccggta ccatataaac ctctgattaa acatggcgcc
    32401 atccaccacc atcctaaacc agctggccaa aacctgcccg ccggctatac actgcaggga
    32461 accgggactg gaacaatgac agtggagagc ccaggactcg taaccatgga tcatcatgct
    32521 cgtcatgata tcaatgttgg cacaacacag gcacacgtgc atacacttcc tcaggattac
    32581 aagctcctcc cgcgttagaa ccatatccca gggaacaacc cattcctgaa tcagcgtaaa
    32641 tcccacactg cagggaagac ctcgcacgta actcacgttg tgcattgtca aagtgttaca
    32701 ttcgggcagc agcggatgat cctccagtat ggtagcgcgg gtttctgtct caaaaggagg
    32761 tagacgatcc ctactgtacg gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt
    32821 catgccaaat ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt
    32881 gacaaacaga tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt
    32941 atatccactc tctcaaagca tccaggcgcc ccctggcttc gggttctatg taaactcctt
    33001 catgcgccgc tgccctgata acatccacca ccgcagaata agccacaccc agccaaccta
    33061 cacattcgtt ctgcgagtca cacacgggag gagcgggaag agctggaaga accatgtttt
    33121 tttttttatt ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc
    33181 tcccctccgg tggcgtggtc aaactctaca gccaaagaac agataatggc atttgtaaga
    33241 tgttgcacaa tggcttccaa aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta
    33301 aacccttcag ggtgaatctc ctctataaac attccagcac cttcaaccat gcccaaataa
    33361 ttctcatctc gccaccttct caatatatct ctaagcaaat cccgaatatt aagtccggcc
    33421 attgtaaaaa tctgctccag agcgccctcc accttcagcc tcaagcagcg aatcatgatt
    33481 gcaaaaattc aggttcctca cagacctgta taagattcaa aagcggaaca ttaacaaaaa
    33541 taccgcgatc ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac
    33601 ggaccagcgc ggccacttcc ccgccaggaa ccttgacaaa agaacccaca ctgattatga
    33661 cacgcatact cggagctatg ctaaccagcg tagccccgat gtaagctttg ttgcatgggc
    33721 ggcgatataa aatgcaaggt gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa
    33781 agcacatcgt agtcatgctc atgcagataa aggcaggtaa gctccggaac caccacagaa
    33841 aaagacacca tttttctctc aaacatgtct gcgggtttct gcataaacac aaaataaaat
    33901 aacaaaaaaa catttaaaca ttagaagcct gtcttacaac aggaaaaaca acccttataa
    33961 gcataagacg gactacggcc atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa
    34021 aaagcaccac cgacagctcc tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca
    34081 catcaggttg attcatcggt cagtgctaaa aagcgaccga aatagcccgg gggaatacat
    34141 acccgcaggc gtagagacaa cattacagcc cccataggag gtataacaaa attaatagga
    34201 gagaaaaaca cataaacacc tgaaaaaccc tcctgcctag gcaaaatagc accctcccgc
    34261 tccagaacaa catacagcgc ttcacagcgg cagcctaaca gtcagcctta ccagtaaaaa
    34321 agaaaaccta ttaaaaaaac accactcgac acggcaccag ctcaatcagt cacagtgtaa
    34381 aaaagggcca agtgcagagc gagtatatat aggactaaaa aatgacgtaa cggttaaagt
    34441 ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc cagaaacgaa agccaaaaaa
    34501 cccacaactt cctcaaatcg tcacttccgt tttcccacgt tacgtaactt cccattttaa
    34561 gaaaactaca attcccaaca catacaagtt actccgccct aaaacctacg tcacccgccc
    34621 cgttcccacg ccccgcgcca cgtcacaaac tccaccccct cattatcata ttggcttcaa
    34681 tccaaaataa ggtatattat tgatgat
//

质粒菌株产品操作说明书
一、扩增流程
         收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
         1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              ①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
              ②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
              ③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
              ④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
              ⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
              ⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
        2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
              四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
        3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
              穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
        4、菌落平板（冰袋运输，存于4℃，保质期7天）
              直接挑取单菌落至液体培养基中。
        5、液体质粒（冰袋运输，存于-20℃，保质期90天）
              单独提取的液体质粒收到后可直接使用。
        6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。

二、转化图片

P3342/pCMV-SPORT6-RAP1B人源基因质粒

P3343/pCMV-SPORT6-TPT1人源基因质粒

P3344/pCMV-SPORT6-SDR39U1人源基因质粒

P3345/pCMV-SPORT6-BABAM2人源基因质粒

P3346/pCMV-SPORT6-DDX56（1同义突变）人源基因质粒

P3347/pCMV-SPORT6-CDKN1B-G326T人源基因质粒

P3348/pCMV-SPORT6-ZNF207（多了一碱基）人源基因质粒

P3349/pCMV-SPORT6-DCTPP1人源基因质粒

P3350/pCMV-SPORT6-ZNF174（1同义突变）人源基因质粒

P3351/pCMV-SPORT6-MFAP3L人源基因质粒

P3352/pCMV-SPORT6-PGAM5(1同义突变1点突变）人源基因质粒