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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1602973-37-4
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N
streptomycin, 37o C. 3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech, 08-110). Stored as 250 ul aliquots in 15 ml conical tubes at -80o C. Keep it cold all the time otherwise it will form a gel. 4. F12K medium (no additives), for diluting
Incubate sections for 10-60 minutes at 37C Wash well in distilled and then tap water Counterstain, with 2% Methyl green for 15-30 seconds (haematoxylin can be used as an alternative) Wash Dehydrate, clear . Mount
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