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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
53885-35-1
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Partial proteolysis with Staph. aureus V8 protease
Materials: Overlay/Rehydration buffer: 125 mM Tris-HCl, pH 6.8 1mM EDTA 0.1% SDS 1mM 2-b-mercaptoethanol 30% glycerol some Bromophenol blue For 1 ml, you will need 0.35 ml of 2X buffer 0.30 ml H20
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
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