- ￥100 - 1000
- Xybio
- 上海
- P0722
- 2025年07月15日
9 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
pCDNA3.1-Laconic
- 库存：
100
- 供应商：
上海烜雅生物科技有限公司
- 规格：
干粉/液体
基本信息
名称：pcDNA3.1-Laconic乳酸传感质粒
别称: pCDNA3.1-Laconic
质粒属性
质粒简介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,
质粒图谱
质粒序列
LOCUS Exported 7648 bp ds-DNA circular SYN 05-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7648)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7648
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 987..1694
/codon_start=1
/product="monomeric teal (cyan) fluorescent protein (Ai et
al., 2006)"
/note="mTFP1"
/note="mammalian codon-optimized"
/translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
VARNSTDGMDELYK"
CDS 2496..3215
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/note="mVenus"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 3248..3472
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 3518..3946
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3960..4289
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 4140..4275
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 4356..5150
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATC-PFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5324..5445
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(5494..5510)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5518..5534
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5542..5572)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5587..5608
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5896..6481)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6652..7512)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7513..7617)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
961 accacactgg actagtggat ccgaacatgg tgagcaaggg cgaggagacc acaatgggcg
1021 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
1081 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
1141 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
1201 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
1261 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
1321 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
1381 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
1441 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
1501 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
1561 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
1621 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
1681 acgagctgta caagagatct ggtaccatga ttgttttacc cagacgcctg tcagacgagg
1741 ttgccgatcg tgtgcgggcg ctgattgatg aaaaaaacct ggaagcgggc atgaagttgc
1801 ccgctgagcg ccaactggcg atgcaactcg gcgtatcacg taattcactg cgcgaggcgc
1861 tggcaaaact ggtgagtgaa ggcgtgctgc tcagtcgacg cggcggcggg acgtttattc
1921 gctggcgtca tgacacatgg tcggagcaaa acatcgtcca gccgctaaaa acactgatgg
1981 ccgatgatcc ggattacagt ttcgatattc tggaagcccg ctacgccatt gaagccagca
2041 ccgcatggca tgcggcaatg cgcgccacac ctggcgacaa agaaaagatt cagctttgct
2101 ttgaagcaac gctaagtgaa gacccggata tcgcctcaca agcggacgtt cgttttcatc
2161 tggcgattgc cgaagcctca cataacatcg tgctgctgca aaccatgcgc ggtttcttcg
2221 atgtcctgca atcctcagtg aagcatagcc gtcagcggat gtatctggtg ccaccggttt
2281 tttcacaact gaccgaacaa catcaggctg tcattgacgc catttttgcc ggtgatgctg
2341 acggggcgcg taaagcaatg atggcgcacc ttagttttgt tcacaccacc atgaaacgat
2401 tcgatgaaga tcaggctcgc cacgcacgga ttacccgcct gcccggtgag cataatgagc
2461 attcgaggga gaaaaacgca cttaagagat ctaccatggt gagcaagggc gaggagctgt
2521 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
2581 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
2641 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
2701 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
2761 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
2821 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
2881 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
2941 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
3001 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
3061 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga
3121 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
3181 ggatcactct cggcatggac gagctgtaca agtaaaagct taagtttaaa ccgctgatca
3241 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
3301 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
3361 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
3421 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
3481 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
3541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
3601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
3661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
3721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
3781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
3841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
3901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
3961 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4021 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4081 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4141 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4201 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
4261 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
4321 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
4381 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
4441 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
4501 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
4561 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
4621 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
4681 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
4741 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
4801 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
4861 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
4921 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
4981 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
5041 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
5101 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
5161 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
5221 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
5281 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
5461 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
5521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
5581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
5641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
5701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
5761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
5821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
5881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
5941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
6001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
6061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
6121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
6181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
6241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
6301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
6361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
6421 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
7621 atttccccga aaagtgccac ctgacgtc
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
名称：pcDNA3.1-Laconic乳酸传感质粒
别称: pCDNA3.1-Laconic
| 启动子: | CMV |
|---|---|
| 复制子: | pUC |
| 终止子: | SV40 poly(A) signal,bGH poly(A) signal |
| 质粒分类: | 哺乳细胞，乳酸生物传感器 |
| 质粒大小: | 7648bp |
| 原核抗性: | Amp |
| 筛选标记: | G418 |
| 克隆菌株: | DH5a |
| 培养条件: | 37度 |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物: | BGH:TAGAAGGCACAGTCGAGG |
质粒属性
| 载体宿主： | 哺乳细胞 |
|---|---|
| 载体用途： | 蛋白表达 |
| 基因种属： | |
| 基因类型： | ORF |
| 原核抗性： | Amp |
| 真核抗性： | Neo/G418 |
| 荧光蛋白： |
质粒简介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,
质粒图谱
质粒序列
LOCUS Exported 7648 bp ds-DNA circular SYN 05-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7648)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7648
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 987..1694
/codon_start=1
/product="monomeric teal (cyan) fluorescent protein (Ai et
al., 2006)"
/note="mTFP1"
/note="mammalian codon-optimized"
/translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
VARNSTDGMDELYK"
CDS 2496..3215
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/note="mVenus"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 3248..3472
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 3518..3946
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3960..4289
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 4140..4275
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 4356..5150
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATC-PFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5324..5445
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(5494..5510)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5518..5534
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5542..5572)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5587..5608
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5896..6481)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6652..7512)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7513..7617)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
961 accacactgg actagtggat ccgaacatgg tgagcaaggg cgaggagacc acaatgggcg
1021 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
1081 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
1141 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
1201 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
1261 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
1321 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
1381 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
1441 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
1501 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
1561 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
1621 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
1681 acgagctgta caagagatct ggtaccatga ttgttttacc cagacgcctg tcagacgagg
1741 ttgccgatcg tgtgcgggcg ctgattgatg aaaaaaacct ggaagcgggc atgaagttgc
1801 ccgctgagcg ccaactggcg atgcaactcg gcgtatcacg taattcactg cgcgaggcgc
1861 tggcaaaact ggtgagtgaa ggcgtgctgc tcagtcgacg cggcggcggg acgtttattc
1921 gctggcgtca tgacacatgg tcggagcaaa acatcgtcca gccgctaaaa acactgatgg
1981 ccgatgatcc ggattacagt ttcgatattc tggaagcccg ctacgccatt gaagccagca
2041 ccgcatggca tgcggcaatg cgcgccacac ctggcgacaa agaaaagatt cagctttgct
2101 ttgaagcaac gctaagtgaa gacccggata tcgcctcaca agcggacgtt cgttttcatc
2161 tggcgattgc cgaagcctca cataacatcg tgctgctgca aaccatgcgc ggtttcttcg
2221 atgtcctgca atcctcagtg aagcatagcc gtcagcggat gtatctggtg ccaccggttt
2281 tttcacaact gaccgaacaa catcaggctg tcattgacgc catttttgcc ggtgatgctg
2341 acggggcgcg taaagcaatg atggcgcacc ttagttttgt tcacaccacc atgaaacgat
2401 tcgatgaaga tcaggctcgc cacgcacgga ttacccgcct gcccggtgag cataatgagc
2461 attcgaggga gaaaaacgca cttaagagat ctaccatggt gagcaagggc gaggagctgt
2521 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
2581 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
2641 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
2701 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
2761 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
2821 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
2881 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
2941 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
3001 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
3061 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga
3121 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
3181 ggatcactct cggcatggac gagctgtaca agtaaaagct taagtttaaa ccgctgatca
3241 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
3301 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
3361 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
3421 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
3481 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
3541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
3601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
3661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
3721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
3781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
3841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
3901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
3961 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4021 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4081 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4141 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4201 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
4261 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
4321 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
4381 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
4441 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
4501 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
4561 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
4621 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
4681 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
4741 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
4801 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
4861 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
4921 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
4981 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
5041 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
5101 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
5161 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
5221 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
5281 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
5461 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
5521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
5581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
5641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
5701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
5761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
5821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
5881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
5941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
6001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
6061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
6121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
6181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
6241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
6301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
6361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
6421 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
7621 atttccccga aaagtgccac ctgacgtc
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
| P3274/pCMV-SPORT6-XBP1人源基因质粒 |
| P3275/pCMV-SPORT6-ICA1L人源基因质粒 |
| P3276/pCMV-SPORT6-TNFAIP1人源基因质粒 |
| P3277/pCMV-SPORT6-CCDC22（1同义突变）人源基因质粒 |
| P3278/pCMV-SPORT6-TCF25（1同义突变）人源基因质粒 |
| P3279/pCMV-SPORT6-STAP2（1点突变1同义突变）人源基因质粒 |
| P3280/pCMV-SPORT6-YPEL5人源基因质粒 |
| P3281/pCMV-SPORT6-LIMA1（294-2280）人源基因质粒 |
| P3282/pCMV-SPORT6-CDK7人源基因质粒 |
| P3283/pCMV-SPORT6-COMMD4人源基因质粒 |
| P3284/pCMV-SPORT6-H1F0人源基因质粒 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
pmCherry-C1 哺乳细胞质粒 Amp http://www.yrgene.com/product/vector/15552 pCDNA3 哺乳细胞质粒 Amp pCDNA3-HA 哺乳细胞质粒 Amp
。同时，由于发酵工业是大规模的生产，微生物传感器其成本低设备简单的特点使其具有极大的优势。 (1). 原材料及代谢产物的测定 微生物传感器可用于原材料如糖蜜、乙酸等的测定，代谢产物如头孢霉素、谷氨酸、甲酸、甲烷、醇类、青霉素、乳酸等的测定。测量的原理基本上都是用适合的微生物电极与氧电极组成，利用微生物的同化作用耗氧，通过测量氧电极电流的变化量来测量氧气的减少量，从而达到测量底物浓度的目的。 在各种原材料中葡萄糖的测定对过程控制尤其重要，用荧光假单胞菌（Psoudomonas
( 1 ) PRSETB 细菌表达质粒（Invitrogen ) : 用于 Ni-次氮基三乙酸（NTA ) 琼脂糖蛋白纯化的 His-tag；用于选择的氨苄青霉素和用于诱导蛋白质表达的异丙基-β-D-硫代半乳糖吡喃糖苷（IPTG )。 ( 2 ) pcDNA3 哺乳动物瞬时表达质粒（Invitrogen ) : 巨细胞病毒启动子和用于细菌选择的氨苄青霉素抗性基因。 2.2 生物化学和生物物理鉴定 ( 1 ) Luria-Bertani ( LB ) 培养基：10 g 胰蛋白胨，5 g 酵母
pcDNA3.1-Laconic乳酸传感质粒
￥100 - 1000
