Goat Anti-Human IgG Secondary

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody

using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).

Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）

and add the Cy5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µ l. Incubate for 4 hours at 4℃ Wash once with cold cyclin staining buffer and once with cold PBS/azide. Resuspend the cells in propidium