Biotinylated Human IL-3RA (C-Fc-Avi) (CY01)
产品说明(Description)
Biotinylated Recombinant Human Interleukin-3 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Thr19-Arg305 is expressed with a Fc, Avi tag at the C-terminus.
Accession #: P26951
Known as: Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunitalpha; IL-3R-alpha; IL-3RA; CD123
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Biotin:Protein Ratio The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Bioactivity-BLI:
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Loaded Human IL-3RA-Fc-Avi(Cat#CY01) on Protein A Biosensor, can bind Human IL-3(Cat#CF63) with an affinity constant of 3.74uM as determined in BLI assay. |
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Loaded Human IL-3RA-Fc-Avi(Cat#CY01) on Protein A Biosensor, can bind Human IL-3(Cat#CF63) with an affinity constant of 3.74uM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD123, also known as Interleukin-3 receptor subunit alpha, belongs to the type I cytokine receptor family. In mouse, there are two classes of high-affinity IL3 receptors. One contains an IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors. CD123 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage?committed cells. CD123 is a heterodimer consisting of an alpha and a beta subunit. The alpha subunit alone binds IL?3 with low affinity. The beta subunit does not bind IL?3 by itself but is required for the high?affinity binding of IL?3 to the heterodimeric receptor complex.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY.