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文献和实验The α1B -adrenergic receptor (α1B -AR) is a G-protein-coupled receptor that plays a key role in the sympathetic regulation of a variety of physiological processes, such as cardiac and smooth muscle contractility, contraction of the spleen, liver
Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells
During microglia activation the levels of active caspase-3, caspase-7 and caspase-8 are increased, which leads to the transcription of proinflammatory cytokines and factors. As such, the induction of caspase activity in microglia can be used
Immunoblotting for Active Caspase-1
Immunoblotting for caspase-1 is the gold-standard method of detecting inflammasome activation. In contrast to IL-1β-based readouts, it can be used in an experimental setup independent of de novo gene expression. Here, we present protocols
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