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文献和实验A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
us to functionally express V2Rs on the surface of heterologous cells. In this chapter we describe protocols for maintenance and expansion of the R24 cell line and functional assays for V2Rs using these cells.
Functional Stem Cell Integration Assessed by Organotypic Slice Cultures
or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell?mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells
RNAqueous® MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for survivin (Hs00153353_m1). Data shown here represent duplicate assays from single transfections. Survivin mRNA levels
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