Matrices listed below were spiked with certain level of CYP83b1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP83b1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
95
EDTA plasma(n=5)
92-104
99
heparin plasma(n=5)
85-103
94
线性度
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP83b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.