Matrices listed below were spiked with certain level of HP and the recovery rates were calculated by comparing the measured value to the expected amount of HP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
98
EDTA plasma(n=5)
86-101
93
heparin plasma(n=5)
85-101
92
线性度
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.