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文献和实验The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation
The detection of auto-antibodies detection is a major aspect of patient’s immunomonitoring. Antibodies directed against the heart and kidney Angiotensin II type 1 receptor (AT1 R) play a major role in antibody mediated rejection after heart
Western blotting using ECL reagent
diluted 1:8000 in TBS-T + 1.5% gelatin for 1 hour.6. Repeat step # 4 (wash)7. Prepare ECL solution for detection: mix equal volume of ECL reagent 1 and 2, (1:1) with final volume regarding of 0.125 ml/cm2 (for a mini gel- 4 ml of each reagent).8. Remove
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
microscope. Utilizing the incorporation of fluorescein-labeled nucleotides, the use of fluorescence microscopy is also possible. B) TUNEL in situ procedure 2. PROTOCOLS A.2.1 Materials Bouin's fixative (A1), alcohols
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