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文献和实验Assay to Detect Lipid Peroxidation upon Exposure to Nanoparticles
hydroperoxides, and aldehydes, such as MDA, can be measured via a thiobarbituric acid reactive substances (TBARS) assay. In this assay, which can be performed in cell culture or in cell lysate, MDA combines with thiobarbituric acid (TBA) to form a fluorescent
Lysosomal Acid Lipase: Assay and Purification
Lysosomal acid lipase (LAL, E.C. 3.1.1.13) is a hydrolase involved in the intracellular degradation of lipoproteins. LAL was first described in fibroblasts (1 ), and since then found in all analyzed tissues with the exception of erythrocytes
The thiobarbituric acid (TBA) assay was developed to quantitatively determine lipid peroxidation for aldehydic compounds in biological matrices. Kohn and Liversedge introduced this methodology in 1944 ( 1 , 2 ). Since its introduction, the TBA
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