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文献和实验Gel Mobility Shift Assay for transcription factor binding
Gel Mobility Shift Assay for transcription factor binding Author: Hueley Lab Source: Contributed by Riddhish Shah, Hueley Lab Abstract: Protocol can be used
Major interfering agents: Detergents, nucleic acids, particulates, lipid droplets Absorbance at 205 nm Range: Roughly 1 to 100 micrograms Volume: Depends on cuvette - volumes range from 200 microliters to 3 ml or greater
to microfuge tube, vortex, and ice for another 10min.) 6) Spin at 14000rpm (~16000xg) for 10min at 4OC. 7) Transfer supernatant to new, pre-chilled microfuge tube. 8) Use 5-10 μl for protein assay, or mix 20 μl with 20 μl 2X sample buffer (or 30 μl
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