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文献和实验100 mM Tris, pH 8.3, 100 mM Tricine, 0.1% SDS Peptide analysis, small protein SDS-PAGE 10x IEF anode buffer 7 mM phosphoric acid Analytical IEF 10x IEF cathode buffer 20 mM lysine, 20 mM arginine Analytical IEF 10x zymogram renaturation
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
:20 microliter binding reaction:4 microliters 5X binding buffer0.2 microliters 0.1 M DTT2000-5000 cpmlabeled DNA0.125 micrograms p[dG-dC]H2O to 20 microliters final volumeAdd proteins to reaction last.Incubate protein and DNA at room temperature
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
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