Biotinylated Human IL-1a (C-Avi) (CY81)
产品说明(Description)
Biotinylated Recombinant Human Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser113-Ala271 is expressed with a Avi tag at the C-terminus.
Accession #: P01583
Known as: Interleukin-1 Alpha; IL-1 Alpha; Hematopoietin-1; IL1A; IL1F1; IL-1a
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Biotin:Protein Ratio The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Interleukin-1 alpha (IL1 α ) is a cytokine member of the interleukin-1 family. IL-1 consists of two distinct forms: IL1 α and IL1 β that recognize the same cell surface receptors but are distinct proteins with approximately 25% amino acid sequence identity. IL1α is constitutively produced by epithelial cells and plays an essential role in maintenance of skin barrier function. Upon stimulation, a wide variety of cells including osteoblasts, monocytes, macrophages can be induced to express IL1 α . IL1 α possesses a wide range of metabolic, physiological, haematopoietic activities, and is critically involved in the regulation of the immune responses and inflammatory responses.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY