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文献和实验Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia
Immunohistochemistry for pimonidazole adducts serves to define hypoxia within tissues. For this purpose, pimonidazole is delivered in vivo, binds to thiol groups at oxygen tensions below 10 mmHg, and is visualized with help of commercially
Immunohistochemical Detection of Tumour Hypoxia
-1) and the exogenous compound pimonidazole (Pim). Immunohistochemistry is routinely used both diagnostically and in research to label and identify specific cellular proteins of interest. The methods described here enable staining of hypoxic cells and tissue in formalin
Hypoxia and Visualization of the Stem Cell Niche
45 for Hematopoietic Stem Cells HSC). However, a universal marker of hypoxia is Hypoxia-Inducible Factor-1, HIF-1, which is stabilized by low ppO2 and acts as a transcription factor to regulate a vast array of genes downstream. HIF-1, together with pimonidazole
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