Laemmli Buffer Stock Solution

PAGE-SDS Laemmli Protocol

  Stock Solutions 1) 1.5M TrisHCl pH 8.3 + 0.4% SDS (adjust pH before you add the SDS). Keep RT. 2) 30% Acrylamide 0.8% Methylene bis Acrylamide or 40% Acrylamide / Methylene bis Acrylamide (ratio: 37.5:1). Keep 4°C 3) 0.5M TrisHCl pH

Stock solutions for tissue culture

inhibit cell dispersal. Tryp/TD is 0.25% Bacto trypsin in TD. It is stored frozen. PBS is a phosphate-buffered physiological saline solution. It is useful if you want to avoid Tris buffer. Like Tris, it contains calcium and magnesium. There is a rarely

Stock solutions for tissue culture

inhibit cell dispersal. Tryp/TD is 0.25% Bacto trypsin in TD. It is stored frozen. PBS is a phosphate-buffered physiological saline solution. It is useful if you want to avoid Tris buffer. Like Tris, it contains calcium and magnesium