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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Actinomycin D (AD) stock solution (1 mg/mL) 7-aminoactinomycin D (7-AAD) stock solution (1 mg/mL) Antibody staining solution Human cells of interest, fresh or cultured (or immortalized human cell lines) Monoclonal antibodies (mAb
【共享】流式 Overview of Flow Cytometry
the saturating concentration of the reagent needed for ananalytical run (UNIT 5.3). For 1 × 106 cells in a total reaction volume of 50 µl, ∼0.125 to 0.5 µg monoclonal antibody must be added to achieve saturation (final concentration in reaction is 2.5 to 10 µg/ml
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