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文献和实验make an Antibody against a Peptides
Carriers Figure 2. EDC-Mediated Peptide-Carrier Conjugation 上一篇:Purification Of Anti Peptide Antibody 下一篇:Polyclonal Antibody Production
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
b-Gal Staining of Murine Embryos
Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (28,4g/l) Phosphate buffer : 23ml of 0.2M NaH2
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