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文献和实验, benzoylecgonine-d3, ecgonine methyl ester-d3, and cocaethylene-d3 and buffered with phosphate buffer. The drugs in the sample are extracted by cation-exchange solid phase extraction. The drugs from the solid phase cartridge are eluted and the eluent is dried
致癌助催剂(promoter)。系从大戟科(Euphorbiaceae)植物巴豆(Croton tiglium)种子搾取获得的巴豆油中所含的促致癌活性成分。也称为TPA(12-0-四癸酰基佛博尔13-乙酸)或PMA(佛博尔豆蔻乙酸)。
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
,collect 10 drop fractions. 13.Collect fluorescent fractions in the void volume,measure volume in a volumetric conical tube. 14.Polymerize actin by adding KCl to 100 mM and MgCl2 to 2 mM.Incubate for 30-60 min at room temperature. 15.Centrifuge in a 50Ti
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