Matrices listed below were spiked with certain level of FN and the recovery rates were calculated by comparing the measured value to the expected amount of FN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
97
EDTA plasma(n=5)
86-104
93
heparin plasma(n=5)
90-105
96
线性度
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.