Recombinant Human CD3D&CD3E He

Recombinant Human CD3D&CD3E Heterodimer (C-6His)  (C01S)

产品说明(Description)

Recombinant Human CD3D&CD3E is produced by our Mammalian expression system and the target geneencoding Phe22-Ala105&Asp23-Asp126 is expressed with a 6His tag at the C-terminus.

Accession #: P04234&P07766

Known as: CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制(Quality Control)

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity-BLI:

 

Loaded Anti-Human/Monkey CD3E mAb-Fc(Cat#NC006) on Protein A Biosensor, can bind  Human CD3D&CD3E Heterodimer-His(Cat#C01S) with an affinity constant of 1.13 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY