- 询价
- Biotium
- 美国
- 29081
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
2年
- 英文名：
Streptavidin Conjugates CF®514 29081
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
CF® dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.
- Conjugates with wide choice of CF® dye colors, plus HRP, R-PE, APC, and Alkaline Phosphatase
- Superior CF® dyes are bright, photostable, and water-soluble
- Ideal for flow cytometry, western blotting, and immunofluorescence staining
Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Streptavidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog number |
|---|---|---|---|---|
| CF®350 Streptavidin | CF®350 | 347/448 nm | 1 mg | 29031 |
| CF®405S Streptavidin | CF®405S | 404/431 nm | 1 mg | 29032 |
| CF®405M Streptavidin | CF®405M | 408/452 nm | 1 mg | 29033 |
| CF®405L Streptavidin | CF®405L | 395/545 nm | 1 mg | 29056 |
| CF®430 Streptavidin | CF®430 | 426/498 nm | 1 mg | 29065 |
| CF®440 Streptavidin | CF®440 | 440/515 nm | 1 mg | 29066 |
| CF®488A Streptavidin | CF®488A | 490/515 nm | 1 mg | 29034 |
| CF®514 Streptavidin | CF®514 | 516/548 nm | 1 mg | 29081 |
| CF®532 Streptavidin | CF®532 | 527/558 nm | 1 mg | 29030 |
| CF®543 Streptavidin | CF®543 | 541/560 nm | 1 mg | 29043 |
| CF®555 Streptavidin | CF®555 | 555/565 nm | 1 mg | 29038 |
| CF®568 Streptavidin | CF®568 | 562/583 nm | 1 mg | 29035 |
| CF®583R Streptavidin | CF®583R | 586/609 nm | 1 mg | 29086 |
| CF®594 Streptavidin | CF®594 | 593/614 nm | 1 mg | 29036 |
| CF®633 Streptavidin | CF®633 | 630/650 nm | 1 mg | 29037 |
| CF®640R Streptavidin | CF®640R | 642/662 nm | 1 mg | 29041 |
| CF®647 Streptavidin | CF®647 | 650/665 nm | 1 mg | 29039 |
| CF®660R Streptavidin | CF®660R | 663/682 nm | 1 mg | 29040 |
| CF®680R Streptavidin | CF®680R | 680/701 nm | 1 mg | 29072 |
| Alkaline Phosphatase Streptavidin (1 mg/mL) |
Alkaline Phosphatase |
N/A | 100 uL | 29071-100uL |
| 1 mL | 29071-1mL | |||
| HRP Streptavidin | HRP | N/A | 1 mg | 29049 |
| R-PE Streptavidin (0.5 mg/mL) |
R-PE | 496, 546, 565/578 nm | 200 uL | 29044-200uL |
| 1 mL | 29044-1mL | |||
| APC Streptavidin (0.5 mg/mL) |
APC | 650/660 nm | 200 uL | 29048-200uL |
| 1 mL | 29048-1mL |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验自动化。二、生物素—链霉亲和素系统放大的时间分辨荧光分析1988年，出现了一种新的双功能螯合剂，称为4,7-双（氯磺酰基苯基）-1,10-菲咯啉-2,9-二羧酸［4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid,BCPDA］，为了增加生物放大又把生物素-亲合素系统（biotin-avidin system,BAS）引入免疫反应系统。本分析系统的基本原理是：固相McAb与抗原反应后，再加入生物素化McAb，形成
plate well，参考步骤 ３（第 ２ 天）。16. 用 ０。５％ BSA in １ × PBS 或者 ０。５％ FCS（胎牛血清）in １ × PBS 稀释链霉亲和素 －HRP（streptavidin－HRP），每孔加入 １００ μl，室温孵育至少 １ h。注意 １：根据显色底物来决定过氧化物酶结合物（peroxidase conjugate）的稀释度。 底物为 AEC，推荐 １：100 稀释度。底物为 TMB，推荐 １：500～１：1000 稀释度。注意 ２：HRP 结合物（HRP－
应用而建立的一种抗原检测系统。 在免疫PCR中，一个中介分子同时具有与DNA、抗体分子特异结合的能力。其一端连接DNA（标记物），另一端连接抗原-抗体复合物，形成一个特殊的抗原-抗体-DNA连接物。作为标记物的DNA分子可用PCR的扩增，存在特异的PCR产物应证明作为标记物的DNA分子已特异地与抗原-抗体复合物结合，而且表明了抗原的存在。有人采用SAP（streptavidin protein A）嵌合体作为中介物。它具有双特异性结合能力，一端为链霉亲和素，可结合生物素；另一端为可与IgG
