Nucleosomes, which fold chromosomal DNA, contain two molecules each of the core histones H2A, H2B, H3, and H4. Almost two turns of DNA are wrapped around this octameric core, which represses transcription.{12364} The histone amino termini extend from th
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This assay kit is available in the following size(s) 96 Well - Please inquire
Cayman’s HDAC Cell-Based Assay provides an easy tool for studying HDAC activity modulators in whole cells. By using a cell-permeable HDAC substrate, the activity of various protein lysine-specific deacetylases, including HDAC1-containing complexes, can be measured in intact cells in a simple and homogenous manner. The fluorescence of the deacetylated reaction product can be analyzed with excitation wavelengths between 340-360 nm and emission wavelengths between 440-465 nm. An HDAC inhibitor, trichostatin A, is included for checking specificity of the HDAC reaction. This assay compliments Cayman’s HDAC Activity Assay (Item No. 10011563), which uses a nuclear extract rather than whole cells for the assay. Together, both assays will help to identify whether an inhibitor/activator has a direct effect on the enzyme.